Purification and Characterization of Corrinoid-Compounds from the Dried Powder of an Edible Cyanobacterium, Nostoc commune (Ishikurage)
スポンサーリンク
概要
- 論文の詳細を見る
Vitamin B_<12> content (98.8±5.6 μg/100 g dry weight) of an edible cyanobacterium, Nostoc commune (Ishikurage) was determined by the Lactobacillus delbrueckii ATCC 7830 microbiological method. Bioautography with vitamin B_<12>-dependent Escherichia coli 215 indicated that N. commune contained two (main and minor) corrinoid-compounds. These corrinoid-compounds were purified to homogeneity from the dried algal cells and characterized. The main and minor purified corrinoid-compounds were identified as pseudovitamin B_<12> and vitamin B_<12>, respectively, on the basis of silica gel 60 TLC, C18 reversed-phase HPLC, ^1H NMR spectroscopy, and UV-visible spectroscopy. These results suggest that the bacterial cells are not suitable for use as a vitamin B_<12> source, especially in vegetarians.
著者
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Nakano Y
Dep. Of Applied Biological Chemistry Graduate School Of Life And Environmental Sciences Osaka Prefec
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MIYAMOTO Emi
Department of Pharmacy, Municipal Ikeda Hospital
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NAKANO Yoshihisa
Graduate School of Life and Environmental Sciences of Osaka Prefecture University
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WATANABE Fumio
School of Agricultural, Biological and Environmental Sciences, Faculty of Agriculture, Tottori Unive
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TANIOKA Yuri
Graduate School of Life and Environmental Science, Osaka Prefecture University
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FUJITA Tomoyuki
Graduate School of Agriculture, Shinshu University
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TAKENAKA Hiroyuki
Micro Algae Corporation
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Fujita Tomoyuki
Graduate School Of Agriculture Shinshu University
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Nakano Yoshihisa
Graduate School Of Life And Environmental Science Osaka Prefecture University
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Nakano Yoshihisa
Nutrition Laboratory College Of Agriculture
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Tamura Yoshiyuki
The Second Department Of Internal Medicine School Of Medicine The University Of Tokushima
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Yoshiyuki Tamura
Laboratory Of Nutrition And Food Science Hagoromo University Of International Studies
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Tamura Yoshiyuki
Second Depertment Of Internal Medicine School Of Medicine The University Of Tokushima
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Tanioka Yuri
Department Of Nutrition Junior College Of Tokyo University Of Agriculture
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Miyamoto Emi
Department Of Health And Nutrition Nagasaki International University
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Tokunaga Yoko
Division Of Applied Biological Chemistry Department Of Bioscience And Biotechnology Faculty Of Agric
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Watanabe Fumio
School Of Agricultural Biological And Environmental Sciences Faculty Of Agriculture Tottori Universi
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Watanabe F
Faculty Of Regional Environmental Science Tokyo University Of Agriculture
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Watanabe Fumio
Department Of Oral And Maxillofacial Sciences Gifu University School Of Medicine
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