Identification, Cloning, and Sequencing of the Genes Involved in the Conversion of D,L-2-Amino-Δ^2-Thiazoline-4-Carboxylic Acid to L-Cysteine in Pseudomonas sp. Strain ON-4a(Biochemistry & Molecular Biology)
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概要
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The newly isolated strain Pseudomonas sp. ON-4a converts D,L-2-amino-Δ^2-thiazoline-4-carboxylic acid to L-cysteine via N-carbamoyl-L-cysteine. A genomic DNA fragment from this strain containing the gene(s) encoding enzymes that convert D,L-2-amino-Δ^2-thiazoline-4-carboxylic acid into L-cysteine was cloned in Escherichia coli. Transformants expressing cysteine-forming activity were selected by growth of an E. coli mutant defective in the cysB gene. A positive clone, denoted CM1, carrying the plasmid pCM1 with an insert DNA of approximately 3.4kb was obtained, and the nucleotide sequence of a complementing region was analyzed. Analysis of the sequence found two open reading frames, ORF1 and ORF2, which encoded proteins of 183 and 435 amino acid residues, respectively. E. coli DH5α harboring pTrCM1, which was constructed by inserting the subcloned sequence into an expression vector, expressed two proteins of 25 kDa and 45 kDa. From the analyses of crude extracts of E. coli DH5α carrying deletion derivatives of pTrCM1 by sodium dodecyl sulfatepolyacrylamide gel electrophoresis and by enzymatic activity, it was found that the 25-kDa protein encoded by ORF1 was the enzyme L-2-amino-Δ^2-thiazoline-4-carboxylic acid hydrolase, which catalyzes the conversion of L-2-amino-Δ^2-thiazoline-4-carboxylic acid to N-carbamoyl-L-cysteine, and that the 45-kDa protein encoded by ORF2 was the enzyme N-carbamoyl-L-cysteine amidohydrolase, which catalyzes the conversion of N-carbamoyl-L-cysteine to L-cysteine.
- 社団法人日本農芸化学会の論文
- 2002-05-23
著者
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Narita Megumi
Department Of Biochemistry And Biotechnology Faculty Of Agriculture And Life Science Hirosaki Univer
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Narita Megumi
Department Of Biochemistry And Biotechnology Faculty Of Agriculture And Life Science Hirosaki Unvier
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Mori Kazuyuki
Department of Urology, Hirosaki University Graduate School of Medicine
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OHMACHI Tetsuo
Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki Univ
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NISHINO Mizuka
Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki Univ
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KAWATA Maki
Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki Univ
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EDO Namiko
Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki Univ
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FUNAKI Hiroko
Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki Univ
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TAMURA Yoshiharu
Research Center, Nippon Rikagakuyakuhin Co. Ltd.
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ASADA Yoshihiro
Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki Univ
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Tamura Yoshiyuki
The Second Department Of Internal Medicine School Of Medicine The University Of Tokushima
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Yoshiyuki Tamura
Laboratory Of Nutrition And Food Science Hagoromo University Of International Studies
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Tamura Yoshiyuki
Second Depertment Of Internal Medicine School Of Medicine The University Of Tokushima
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Edo Namiko
Department Of Biochemistry And Biotechnology Faculty Of Agriculture And Life Science Hirosaki Univer
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Kawata Maki
Department Of Biochemistry And Biotechnology Faculty Of Agriculture And Life Science Hirosaki Univer
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Tanioka Yuri
Department Of Nutrition Junior College Of Tokyo University Of Agriculture
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Tokunaga Yoko
Division Of Applied Biological Chemistry Department Of Bioscience And Biotechnology Faculty Of Agric
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Funaki Hiroko
Department Of Biochemistry And Biotechnology Faculty Of Agriculture And Life Science Hirosaki Univer
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Mori Kazuyuki
Department Of Biochemistry And Biotechnology Faculty Of Agriculture And Life Science Hirosaki Univer
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Asada Y
Department Of Biochemistry And Biotechnology Faculty Of Agriculture And Life Science Hirosaki Univer
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Ohmachi T
Department Of Biochemistry And Biotechnology Faculty Of Agriculture And Life Science Hirosaki Univer
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Ohmachi Tetsuo
Department Of Biochemistry And Biotechnology Faculty Of Agriculture And Life Science Hirosaki Univer
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Nishino Mizuka
Department Of Biochemistry And Biotechnology Faculty Of Agriculture And Life Science Hirosaki Univer
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Asada Yoshihiro
Department Of Agricultural Chemistry Faculty Of Agriculture Ibaraki University
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