Anti-tumor eff ect of nano-particulated bacillus Calmette-Guerin（BCG）cell wall complex on bladder cancer cell line

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Bacillus Calmette-Guerin（ BCG） has been widely accepted as an eff ective treatment for CIS and superfi cialcarcinoma of the bladder. However, it has considerable side eff ects and toxicity. We thought if eff ective bacterial cellwall elements as substitutes for live bacteria were identifi ed, useful treatment with lower toxicity while maintainingstrong anti-tumor eff ects might be possible. For these reasons we generated a nano-particulated BCG complex, whichdoes not contain live bacteria. Here, we present its direct in vitro anti-tumor eff ect on bladder cancer cell lines. Tokyo172 BCG sub-strain was disrupted by French press with monitoring the particle distribution by the particle analyzer.After removing the not-disrupted bacteria by centrifuge at 6,800×g, supernatant was centrifuged at 18,000×g. Then thesupernatant（ Sup） and the precipitate（ CW） were lyophilyzed to obtain nano-particulated BCG complex. Bladder cancercell lines, J82 and KK47, were co-cultured with BCG, Sup, CW or Sup+CW（ mix） for 5 days, then viable cell numberswere counted. In J82 cells, when separately added to the culture medium, both CW and Sup reduced cell number toabout 70% of control cells. While they were mixed together, they reduced cell number equally compared with BCG; 59.2%（mix） vs 60.2%（ BCG） in J82 cells, 67.3% vs 68.8% in KK47 cells. These preliminary in vitro experiments demonstratedthe identical direct anti-tumor eff ect of nano-particulated BCG to that of live BCG. In vivo tumor assays are warrantedfor clinical application of nano-particulated BCG.
弘前大学の論文

Anti-tumor eff ect of nano-particulated bacillus Calmette-Guerin（BCG）cell wall complex on bladder cancer cell line

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