Glutamic Acid Independent Production of Poly(γ-glutamic acid) by Bacillus subtilis TAM-4

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概要

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• A bacterium that produced a large amount of poly(γ-glutamic acid) (PGA) when it was grown aerobically in a culture medium containing ammonium salt and sugar as sources of nitrogen and carbon, respectively, was isolated from soil. The bacterium, strain TAM-4, was classified as Bacillus subtilis. The maximum PGA production (22.1 mg/ml) was obtained when it was grown in a medium containing 1.8% ammonium chloride and 7.5% fructose at 30℃ for 96 h with shaking. Some properties of the PGA obtained at different times of cultivation were investigated by gel permeation chromatography, SDS-PAGE, and measurement of viscosity, and calculation of the D/L ratio of glutamic acid constituting PGA. The results suggested that PGA was elongated with no changes in the diastereoisomer ratio in the molecule.

• 社団法人日本農芸化学会の論文
• 1996-08-23

著者

• Tanaka Takeshi

  Department Of Cardiology The Heart Institute Of Japan

• OHMACHI Tetsuo

  Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki Univ

• ASADA Yoshihiro

  Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki Univ

• Ito Yoshihito

  Department Of Science Of Bioresources Faculty Of Agriculture Hirosaki University

• Ito Yoshihito

  Department Of Environmental Science And Technology Tokyo Institute Of Technology

• Asada Y

  Department Of Biochemistry And Biotechnology Faculty Of Agriculture And Life Science Hirosaki Univer

• Ohmachi T

  Department Of Biochemistry And Biotechnology Faculty Of Agriculture And Life Science Hirosaki Univer

• Ohmachi Tetsuo

  Department Of Biochemistry And Biotechnology Faculty Of Agriculture And Life Science Hirosaki Univer

• Asada Yoshihiro

  Department Of Agricultural Chemistry Faculty Of Agriculture Ibaraki University

• Tanaka Takeshi

  Department Of Biomedical Chemistry Graduate School Of Medicine The University Of Tokyo

• Tanaka Takeshi

  Department Of Science Of Bioresources Faculty Of Agriculture Hirosaki University

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