Production of γ-Polyglutamic Acid by Bacillus licheniformis A35 under Denitrifying Conditions(Microbiology & Fermentation Industry)
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概要
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The possibility of producing amino acids under conditions of denitrification, i.e., with nitrate respiration as an energy-supplying system, was investigated using denitrifying bacteria. We found that Bacillus licheniformis A35 accumulated γ-polyglutamic acid (PGA) to a concentration of 8mg per ml in a medium containing glucose and ammonium chloride or glutamic acid under denitrifying conditions. The bacterium also produced PGA aerobically. Thus, we found the de novo synthesis of PGA with B. licheniformis A35. The optimum conditions for PGA production were determined. The purified PGA was found to be composed solely of glutamic acid ; both D- and L-glutamic acid, in a molar ratio of 4 : 1. The molecular weight of PGA was about 3x10^5. Other bacilli tested produced some free amino acids but not under nitrate-respiration conditions, although some of them did produce PGA aerobically from L-glutamic acid.
- 社団法人日本農芸化学会の論文
- 1989-09-23
著者
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Aida Tokujiro
Department Of Agricultural Chemistry Faculty Of Agriculture Ibaraki University
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Asada Yoshihiro
Department Of Agricultural Chemistry Faculty Of Agriculture Ibaraki University
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CHENG Cheng
Department of Agricultural Chemistry, Faculty of Agriculture, Ibaraki University
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Cheng C
Nagoya Univ. Nagoya Jpn
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