N-Carbamoyl-L-Cysteine as an Intermediate in the Bioconversion from D, L-2-Amino-Δ^2-Thiazoline-4-Carboxylic Acid to L-Cysteine by Pseudomonas sp. ON-4a

概要

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We investigated the conversion of D, L-2-amino-Δ^2-thiazoline-4-carboxylic acid (D, L-ATC) to L-cysteine with Pseudomonas sp. ON-4a, an ATC-assimilating bacterium. Cysteine and N-carbamoylcysteine (NCC), but not S-carbamoylcysteine (SCC), were produced from D, L-ATC by a cell-free extract from the strain. These products were isolated from the reaction mixture and then identified as the L-form. Similar results were obtained with P.putida AJ3865 and unidentified strain TG-3,an ATC-assimilating bacteria. It became clear that L-NCC is an intermediate in the conversion of D, L-ATC to L-cysteine in these Pseudomonas strains. Furthermore, it was suggested that these bacteria have L-ATC hydrolase and L-NCC amidohydrolase.
社団法人日本農芸化学会の論文
1998-11-23