Purification and Properties of Two Isozymes of γ-Glutamyltranspeptidase from Bacillus subtilis TAM-4
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概要
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Two isozymes of γ-glutamyltranspeptidase, GGT-A and GGT-B, were purified to elettrophoretic homogeneity from a culture broth of Bacillus subtilis TAM-4, which produces poly(γ-glutamic acid) (PGA) de novo. GGT-A was composed of three subunits with molecular weights of 23,000 (I), 39,000 (II), and 40,000 (III). GGT-B was composed of two subunits with molecular weights of 22,000 (I) and 39,000 (II). The N-terminal amino acid sequences of GGT-A subunit I and GGT-B subunit I were very similar. GGT-A subunit II and GGT-B subunit II had an identical N-terminal amino acid sequence. That of GGT-A subunit III showed no similarity to the other subunits. Both GGTs had similar enzymatic properties (optimum pH and temperature: pH 8.8 and 55℃) but showed a signiticantly different thermal stability at 55℃. Both GGT-A and -B used D-γ-glutamyl-p-nitroanilide as well as the L-isomer as the γ-glutamyl donor and used various amino acids and peptides as the acceptor. It was also found that the PGA produced by the strain was hydrolyzed to glutamic acid by its own GGTs.
- 社団法人日本農芸化学会の論文
- 1997-10-23
著者
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ABE Kazuhiro
Department of Anatomy, Hokkaido University Graduate School of Medicine
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Abe Kazuhiro
Department Of Anatomy Hokkaido University Graduate School Of Medicine
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OHMACHI Tetsuo
Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki Univ
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ASADA Yoshihiro
Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki Univ
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Ito Yoshihito
Department Of Science Of Bioresources Faculty Of Agriculture Hirosaki University
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Ito Yoshihito
Department Of Environmental Science And Technology Tokyo Institute Of Technology
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Asada Y
Department Of Biochemistry And Biotechnology Faculty Of Agriculture And Life Science Hirosaki Univer
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Ohmachi T
Department Of Biochemistry And Biotechnology Faculty Of Agriculture And Life Science Hirosaki Univer
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Ohmachi Tetsuo
Department Of Biochemistry And Biotechnology Faculty Of Agriculture And Life Science Hirosaki Univer
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Asada Yoshihiro
Department Of Agricultural Chemistry Faculty Of Agriculture Ibaraki University
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Abe Kazuhiro
Department Of Science Of Bioresources Faculty Of Agriculture Hirosaki University
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