Surface Structures of Cultured Type 2 Astrocytes Revealed by Atomic Force Microscopy(Neurobiology)
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概要
- 論文の詳細を見る
The incomplete morphological characterization of type 2 astrocytes is in part responsible for the slow progress of studies on these cells. To examine and characterize type 2 astrocytes morphologically, three-dimensional fine structures of the surfaces of type 2 astrocytes cultured from rat cerebella were studied by a combination of atomic force microscopic and immunocytochemical techniques. Atomic force microscopy (AFM) revealed irregular ridge-like structures that form a meshwork distributed throughout the cell body surfaces and the thick processes. These ridges were found to be of two heights (31 nm and 82 nm). This finding indicates two possible configurations responsible for shaping the meshwork : (1) two structures of different thickness are beneath the cell membrane ; and (2) two structures are located at two different depths from the cell membrane. On the other hand, immunocytochemical studies for tubulin and glial fibrillary acidic protein (GFAP) revealed that these cytoskeletal filaments are similarly distributed within the resolution power of a light microscope. However, no detectable structures were obtained by actin staining. The immunocytochemical findings suggest that the AFM-revealed ridges forming the irregular meshwork on the cell surfaces may reflect very fine bundles of tubulin and/or GFAP. Therefore, AFM study, with the help of immunocytochemical study, is a powerful tool for characterizing cell morphology. The results of the present study reveal the first morphological characterization of type 2 astrocytes.
- 社団法人日本動物学会の論文
- 1999-02-15
著者
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伊藤 悦朗
Kagawa School Of Pharmaceutical Science Tokushima Bunri University
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Ito Etsuro
北海道大学 理研究
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Ito Etsuro
Division of Innovative Research, Creative Research Initiative "Sousei", Hokkaido University
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Hatakeyama Dai
Division of Biological Sciences, Graduate School of Science, Hokkaido University
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YAMANE Yukako
Division of Biological Sciences, Graduate School of Science, Hokkaido University
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ABE Kazuhiro
Department of Anatomy, Hokkaido University Graduate School of Medicine
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HAGA Hisashi
Division of Physics, Graduate School of Science, Hokkaido University
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Ito Etsuro
Laboratory Of Animal Behavior And Intelligence Division Of Biological Sciences Graduate School Of Sc
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Ito Etsuro
Advanced Research Center For Human Sciences Waseda University
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Ito E
Divisions Of Biological Sciences Hokkaido University
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Hatakeyama D
Department Of Biological Science And Technology School Of High-technology For Human Welfare Tokai Un
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Hatakeyama Dai
Division Of Biological Sciences Graduate School Of Science Hokkaido University
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Hatakeyama Dai
Divisions Of Biological Sciences Hokkaido University
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Haga Hisashi
Department Of Physics Faculty Of Science Hokkaido University
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Ito Etsuro
Division Of Biological Sciences Graduate School Of Science Hokkaido University
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Abe Kazuhiro
Department Of Anatomy Hokkaido University Graduate School Of Medicine
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ABE Kazuhiro
Hokkaido University School of Medicine
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Ito E
Division Of Innovative Research Creative Research Initiative "sousei" Hokkaido University:
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Haga Hisashi
Division Of Biological Sciences Graduate School Of Science Hokkaido University
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Yamane Yukako
Division Of Biological Sciences Graduate School Of Science Hokkaido University
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Haga Hisashi
Division Of Biological Sciences Graduate School Of Science
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