Efficient Production of Taka-amylase A by Trichoderma viride(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
An efficient heterologous protein production system was developed in Trichoderma viride, a very efficient cellulase producer. An expression vector containing the Taka-amylase A gene from Aspergillus oryzae, which was fused to the strong promoter and signal peptide sequence of the cellobiohydrolase 1 gene (cbh1) of T. viride, and the hygromycin B resistance gene was used to transform protoplasts of T. viride. Using hygromycin B resistance, a frequency of 3 transformants per μg DNA on average was obtained. One transformant showed highly elevated α-amylase production, 1.0 g/l, which was shown to be under the control of the cbh1 gene promoter. Analysis of the chromosomal DNA of the transformant showed the integration of more than one copy of the vector.
- 社団法人日本農芸化学会の論文
- 1991-07-23
著者
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Udaka Shigezo
Department Of Food Science And Technology Faculty Of Agriculture Nagoya University
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Udaka Shigezo
Department Of Brewing And Fermentation Tokyo University Of Agriculture
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CHENG Cheng
Department of Agricultural Chemistry, Faculty of Agriculture, Ibaraki University
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