Molecular Cloning of a Fungal cDNA Encoding Protein Disulfide Isomerase
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概要
- 論文の詳細を見る
Based on the partial amino acid sequences of a protein disulfide isomerase (PDI) from Humicola insolens, two primers were synthesized for reverse transcriptase mediated ploymerase chain reaction (RT-PCR) of a fungal RNA. A 0. 2-kbp fragment around the consensus sequence of PDIs was obtained and used as a probe for screening a fungal cDNA library. A cDNA clone of PDI from H. insolens was isolated and encoded a polypeptide consisting of 505 amino acids, which was characterized by a N-terminal signal sequence composed of 20 amino acids, a consensus sequence (WCGHCK) at two positions, and a C-terminal endoplasmic reticulum retention signal (HDEL). Bacillus brevis harboring an expression plasmid bearing the fungal PDI cDNA was prepared and its culture supernatant showed a signiticant PDI activity. This indicates that glycosylation of a fungal PDI is not essential for the enzymatic activity related to an interchange of disulfide bonds.
- 社団法人日本農芸化学会の論文
- 1994-08-23
著者
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Asami Osamu
Toyota Central Research & Development Labs
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Kajino Tsutomu
Toyota Central Research & Development Labs
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Asami O
Toyota Central Res. & Dev. Labs. Inc. Aichi Jpn
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Kajino T
Toyota Central Research & Development Labs. Inc.
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Kajino Tsutomu
Toyota Central R & D Labs Inc.
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HIRAI Makoto
Development and Differentiation Department, National Institute of Agrobiological Sciences
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HIRAI MASANA
Toyota Central Research & Development Labs., Inc.
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YAMADA YUKIO
Toyota Central Research & Development Labs., Inc.
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UDAKA SHIGEZO
Department of Fermentation Sciences, Tokyo University of Agriculture
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Yamada Yukio
Fine Chemicals Research Laboratories Kaneka Corporation
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Sarai Kiyoko
Toyota Central Research and Development Laboratories Inc.
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Imaeda Takao
Toyota Central Research and Development Laboratories Inc.
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Idekoba Chie
Toyota Central Research and Development Laboratories Inc.
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Yamada Yukio
Mechanical Engineering Laboratory Material Science And Bioengineering Department Aist
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Hirai M
Center For Advanced Material Analysis Tokyo Institute Of Technology
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Udaka S
Faculty Of Applied Bio-science Tokyo University Of Agriculture
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Udaka Shigezo
Department Of Brewing And Fermentation Tokyo University Of Agriculture
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Udaka Shigezo
Dept. Of Food Science And Technology Faculty Of Agriculture Nagoya University
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