A Permease Exhibiting a Dual Role for Lysine and Biotin Uptake in Saccharomyces cerevisiae

スポンサーリンク

概要

• 論文の詳細を見る

• Among Saccharomyces cerevisiae strains each defective in one of 11 amino acid permeases, a lysine permease disruptant (DK) exhibited about 2-fold reductions in maximum cell density and fermentation ability compared to the parent in a synthetic medium. These unusual properties of DK were found to result from the requirement of biotin for growth, in contrast to the parent whose growth was not dependent on external biotin. The rate of ^<14>C-labeled biotin uptake and the intracellular free biotin content of DK were 2-2.5 fold lower than in the parent. We suggest that lysine permease in S.cerevisiae has the ability to transport both lysine and biotin.

• 社団法人日本生物工学会の論文
• 2000-01-25

著者

• Koizumi Yukimichi

  Department of Fermentation Science, Faculty of Applied Bio-Science, Tokyo University of Agriculture

• Kosugi Akihiko

  Department Of Fermentation Science Faculty Of Applied Bio-science Tokyo University Of Agriculture

• Kawamura Yukio

  Department Of Fermentation Science Faculty Of Applied Bio-science Tokyo University Of Agriculture

• Kawamura Y

  Akita Res. Inst. Food And Brewing (arif) Akita

• Kawamura Y

  National Food Res. Inst. Ibaraki Jpn

• UDAKA SHIGEZO

  Department of Fermentation Sciences, Tokyo University of Agriculture

• Kashima Y

  National Inst. Of Advanced Industrial Sci. And Technol. (aist Kansai) Osaka Jpn

• Koizumi Y

  Department Of Fermentation Science Faculty Of Applied Bio-science Tokyo University Of Agriculture

• YAMAGIDA FUJIHARU

  Department of Fermentation Science, Tokyo University of Agriculture

• Yanagida Fujiharu

  Department Of Fermentation Science Faculty Of Applied Bioscience Tokyo University Of Agriculture

• Udaka S

  Faculty Of Applied Bio-science Tokyo University Of Agriculture

• Udaka Shigezo

  Department Of Brewing And Fermentation Tokyo University Of Agriculture

• Koizumi Yukimichi

  Department Of Fermentation Science Faculty Of Applied Bio-science Tokyo University Of Agriculture

• Koizumi Y

  Kyoto Univ. Kyoto Jpn

• Udaka Shigezo

  Dept. Of Food Science And Technology Faculty Of Agriculture Nagoya University

• YANAGIDA Fujiharu

  Department of Brewing and Fermentation, Tokyo University of Agriculture

関連論文

• Cloning and Characterization of clpB in Acetobacter pasteurianus NBRC3283(BREWING AND FOOD TECHNOLOGY)
• Cloning and characterization of grpE in Acetobacter pasteurianus NBRC3283(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Isolation of a Protease-Deficient Mutant of Bacillus brevis and Efficient Secretion of a Fungal Protein Disulfied Isomerase by the Mutant
• Genetic Transformation of Bacillus brevis with Plasmid DNA by Electroporation(Microbiology & Fermentation Industry)
• Potential Use of Bacillus brevis HPD31 for the Production of Foreign Proteins(Microbiology & Fermentation Industry)
• Screening and Characterization of Protein-hyperproducing Bacteria without Detectable Exoprotease Activity(Microbiology & Fermentation Industry)
• Substantially Complete Removal of Three Major Allergenic Soybean Proteins (Gly m Bd 30K, Gly m Bd 28K, and the α-Subunit of Conglycinin) from Soy Protein by Using a Mutant Soybean, Tohoku 124
• Novel Benzacridine Derivative in the Green Pigment from Methyl Caffeate and Butylamine
• Development of Free Radical Products during the Greening Reaction of Caffeic Acid Esters (or Chlorogenic Acid) and a Primary Amino Compound(Food & Nutrition Science)
• Structure of Green Pigment Formed by the Reaction of Caffeic Acid Esters (or Chlorogenic acid) with a Primary Amino Compound(Food & Nutrition Science)
• A Gene Encoding Phosphatidylethanolamine N-Methyltransferase from Acetobacter aceti and Some Properties of its Disruptant(Microbiology & Fermentation Technology)
• MUP1,High Affinity Methionine Permease, is Involved in Cysteine Uptake by Saccharomyces cerevisiae
• The est1 Regulation Depends on the Oxygen Concentration in Acetobacter pasteurianus
• A Permease Exhibiting a Dual Role for Lysine and Biotin Uptake in Saccharomyces cerevisiae
• High Effeciency Transformation of Bacillus brevis by Electroporation
• Protein Disulfide Isomerase Activity of Some Plant Seeds
• Molecular Cloning of a Fungal cDNA Encoding Protein Disulfide Isomerase
• Purification of Protein Disulfide Isomerase from a Thermophilic Fungus
• Substantially Complete Removal of the 34 kDa Allergenic Soybean Protein, Gly m Bd 30 K, from Soy Milk of a Mutant Lacking the α- and α'-Subunits of Conglycinin
• Molecular Cloning of Acid-Stable Glucose Isomerase Gene from Streptomyces olivaceoviridis E-86 by a Simple Two-Step PCR Method, and Its Expression in Escherichia coli
• Identification of Functionally Important Cysteine Residues of the Haman Renin-Binding Protein as the Enzyme N-Acetyl-D-Glucosamine 2-Epimerase 1
• Renin Inhibits N-Acetyl-D-Glucosamine 2-Epimerase (Renin-Binding Protein)^1
• Construction of a Chimeric Shuttle Plasmid via a Heterodimer System : Secretion of an scFv Protein from Bacillus brevis Cells Capable of Inhibiting Hemagglutination
• Random Point Mutation Analysis of the Signal Peptide Cleavage Area of Bacillus stearothermophilus α-Amylase(Microbiology & Fermentation Industry)
• Accumulation and Degradation of Thiamin-binding Protein and Level of Thiamin in Wheat Seeds during Seed Maturation and Germination
• Secretory Production of Erythropoietin and the Extracellular Domain of the Erythropoietin Receptor by Bacillus brevis : Affinity Purification and Characterization
• Purification and Characterizationof Protein Disulfide Isomerase from Soybeam
• Gene Expression in Bacillus brevis under Control of the Lac Repressor-Operator System from Escherichia coli
• Production of Human Epidermal Growth Factor by Bacillus brevis Increased with Use of a Stable Plasmid from B. brevis 481
• 565 Charcteristics of Batch Culture of a Recombinant ___- ___- Producing a Foreign Esterase
• Analysis by Deletion and Site-directed Mutagenesis of Promoters of the Cell Wall Protein Gene Operon in Bacillus brevis 47(Microbiology & Fermentation Industry)
• β-Lactotensin, a Neurotensin NT_2 Agonist, Stimulates Bile Acid Secretion
• Physiological effects and mechanism of Arg-Ile-Tyr, a multifunctional peptide derived from rapeseed protein
• Effects of dietary polyamines on the promotion of mammary tumor in rats.
• Effects of Dietary Polyamines on the Promotion of Mammary Tumor in Rats
• Cloning and Characterization of the dnaKJ Operon in Acetobacter aceti (MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Cloning and Characterization of groESL Operon in Acetobacter aceti
• Role of Intracellular Esterases in the Production of Esters by Acetobacter pasteurianus
• Cloning and Characterization of Ethanol-Regulated Esterase Genes in Acetobacter pasteurianus
• Angiotensin I Converting Enzyme Inhibitory Activities of Various Fermented Foods
• Transepithelial Transport of the Bioactive Tripeptide, Val-Pro-Pro, in Human Intestinal Caco-2 Cell Monolayers(Food & Nutrition Science)
• Purification and Characterization of Intracellular Esterases Related to Ethylacetate Formation in Acetobacter pasteurianus
• Polyamine Content of Ordinary Foodstuffs and Various Fermented Foods
• Efficient Production of Bacillus stearothermophilus α-Amylase in Bacillus brevis by Altering Its Signal Peptide
• Production of Thermostable Clostridium thermohydrosulfuricum Xylose Isomerase in Bacillus brevis(Microbiology & Fermentation Industry)
• Xylose (Glucose) Isomerase Gene from the Thermophile Clostridium thermohydrosulfuricum; Cloning, Sequencing, and Expression in Escherichia coli(Microbiology & Fermentation Industry)
• Efficient Production of N-terminally Truncated Biologically Active Human Interleukin-6 by Bacillus brevis
• Efficient Production of Thermostable Clostridium thermosulfurogenes β-Amylase by Bacillus brevis
• 107 Effective Extracellular Production of Bacillus stearothermophilus Esterase by pH-stat modal Fed-batch Culture of Recombinant Bacillus brevis
• Hydrogen Peroxide Resistance of Acetobacter pasteurianus NBRC3283 and Its Relationship to Acetic Acid Fermentation
• Purification and Properties of Divalent Cation-Dependent Raw-Starch-Digesting α-Amylase from Bacillus sp. B1018
• Characterization of rpoH in Acetobacter pasteurianus NBRC3283(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Secretion of Human Interleukin-2 in Biologically Active Form by Bacillus brevis Directly into Cultute Medium
• BbrPI, an Extracellular Proteinase Inhibitor of Bacillus brevis, Protects Cells from the Attack of Exogenous Proteinase
• Isolation of Peptides from an Enzymatic Hydrolysate of Food Proteins and Characterization of Their Taste Properties
• Optimal Culture Conditions for Production of the Macromolecular Peptide Antibiotics AN-1,AN-3 and AN-7
• Sweetness of Lysozymes
• Efficient Production of Taka-amylase A by Trichoderma viride(Microbiology & Fermentation Industry)
• Activity and Synthesis of β-Galactosidase in Various Lactose Utilizing Bacteria
• Angiotensin I-Converting Enzyme Inhibitory Action of Fish Sauce.
• Detection of Plasmids in Bacillus brevis Species and Introduction of a Plasmid into B. brevis 47, a Protein-producing Bacterium, by Cotransformation
• Stability of a recombinant plasmid containing .ALPHA.-amylase gene in chemostat.
• Clones with New Phenotypes through Transformation of Bacillus brevis 47, a Protein-producing Bacterium

もっと見る 閉じる

スポンサーリンク