Transepithelial Transport of the Bioactive Tripeptide, Val-Pro-Pro, in Human Intestinal Caco-2 Cell Monolayers(Food & Nutrition Science)
スポンサーリンク
概要
- 論文の詳細を見る
Some of the food-derived tripeptides with angiotensin converting enzyme (ACE)-inhibitory activity have been reported to be hypotensive after being orally administered. The mechanism for the intestinal transport of these tripeptides was studied by using monolayer-cultured human intestinal Caco-2 cells which express many enterocyte-like functions including the peptide transporter(PepT1)-mediated transport system. Val-Pro-Pro, an ACE-inhibitory peptide from fermented milk, was used as a model tripeptide. A significant amount of intact Val-Pro-Pro was transported across the Caco-2 cell monolayer. This transport was hardly inhibited by a competitive substrate for PepT1. Since no intact Val-Pro-Pro was detected in the cells, Val-Pro-Pro apically taken by Caco-2 cells via PepT1 was likely to have been quickly hydrolyzed by intracellular peptidases, producing free Val and Pro. These findings suggest that PepT1-mediated transport was not involved in the transepithelial transport of intact Val-Pro-Pro. Paracellular diffusion is suggested to have been the main mechanism for the transport of intact Val-Pro-Pro across the Caco-2 cell monolayer.
- 社団法人日本農芸化学会の論文
- 2002-02-23
著者
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ARAI Soichi
Department of Nutritional Science, Faculty of Applied Bioscience, Tokyo University of Agriculture
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SHIMIZU Makoto
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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Shimizu M
Univ. Tokyo Tokyo Jpn
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Kawamura Yukio
Department Of Fermentation Science Faculty Of Applied Bio-science Tokyo University Of Agriculture
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Arai S
Tokyo Univ. Agriculture Tokyo Jpn
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Arai S
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Arai S
Department Of Applied Biological Chemistry The University Of Tokyo
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Arai Soichi
Department Of Applied Biological Chemistry The University Of Tokyo
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Arai Soichi
Department Of Applied Biological Chemistry Division Of Agriculture And Agricultural Life Sciences Th
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Arai Soichi
Department Of Applied Biological Chemistry Division Of Agriculture And Agricultural Life Science The
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Arai Soichi
Department Of Agricultural Chemistry The University Of Tokyo
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SATAKE Makoto
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Science, The Un
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Satake Makoto
Department Of Applied Biological Chemistry The University Of Tokyo
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Satake Makoto
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Science The Univ
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Arai Soichi
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Arai Soichi
Department Of Nutrition Tokyo University Of Agriculture
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Arai S
Laboratory Of Environmental Biochemistry Graduate School Of Pharmaceutical Sciences Osaka University
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Kawamura Yukio
Department Of Bioengineering Akita Research Institute Offood And Brewing (arif)
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ENJOH Masashi
Department of Applied Biological Chemistry, The University of Tokyo
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NAKAMURA Yasunori
Research and Development Center, The Calpis Co. Ltd.
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TAKANO Toshiaki
Research and Development Center, The Calpis Co. Ltd.
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Enjoh Masashi
Department Of Applied Biological Chemistry The University Of Tokyo
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Shimizu M
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Kawamura Yukio
National Food Res. Inst. Ibaraki Jpn
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Shimizu Makoto
Department Of Applied Biological Chemistry The University Of Tokyo
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Arai Soichi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Nakamura Yasunori
Fac. Of Bioresource Sciences Akita Prefectural Univ.
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Shimizu Makoto
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Shimizu Makoto
Department Of Agricultural Chemistry Faculty Of Engineering The University Of Tokyo
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Kawamura Yukio
Department Of Applied Biological Chemistry Graduate School Of Agriculture Kinki University
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ARAI Soichi
Department of Agricultural Chemicatry, The University of Tokyo
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