Molecular Cloning and Characterization of an α-Amylase from Pichia burtonii 15-1
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概要
- 論文の詳細を見る
An α-amylase secreted by Pichia burtonii 15-1 isolated from a traditional starter murcha of Nepal, named Pichia burtonii α-amylase (PBA), was studied. The gene was cloned and its nucleotide sequence was determined. PBA was deduced to consist of 494 amino acid residues. It shared certain degrees of amino acid sequence identity with other homologous proteins: 60% with Schwanniomyces occidentalis α-amylase, 58% with Saccharomycopsis sp. α-amylase, and 47% with Taka-amylase A from Aspergillus oryzae. A three-dimensional structural model of PBA generated using the known three-dimensional structure of Taka-amylase A as a template suggested high structural similarity between them. Kinetic analysis revealed that the Km values of PBA were lower than those of Taka-amylase A for the oligosaccharides. Although the kcat values of PBA were lower than those of Taka-amylase A for the oligosaccharide substrates, the kcat⁄Km values of PBA were higher.
- 社団法人 日本農芸化学会の論文
- 2007-12-23
著者
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ARAI Soichi
Department of Nutritional Science, Faculty of Applied Bioscience, Tokyo University of Agriculture
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TOKUE Chiyoko
Department of Nutritional Science, Faculty of Applied Bio-Science, Tokyo University of Agriculture
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Shimizu Ibuka
Department Of Food And Nutritional Sciences Graduate School Of Nutritional And Environmental Science
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Tokue C
Tokyo Univ. Agriculture Tokyo Jpn
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Tokue Chiyoko
Department Of Nutritional Science Faculty Of Applied Bio-science Junior College Of Tokyo University
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Arai Soichi
Department Of Nutritional Science Faculty Of Applied Bioscience Tokyo University Of Agriculture
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Arai Soichi
Department Of Agricultural Chemistry The University Of Tokyo
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Arai Soichi
Department Of Nutritional Science Faculty Of Applied Bio-science Tokyo University Of Agriculture
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MURA Kiyoshi
Department of Nutritional Science, Tokyo University of Agriculture
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TAKEUCHI Akiko
Department of Nutritional Science, Faculty of Applied Bio-Science, Tokyo University of Agriculture
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Takeuchi Akiko
Department Of Biological Science Faculty Of Science Nara Women's University
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Takeuchi Akiko
Department Of Nutritional Science Faculty Of Applied Bio-science Tokyo University Of Agriculture
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Shimizu Ibuka
Department Of Nutritional Science Faculty Of Applied Bio-science Tokyo University Of Agriculture
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Arai Soichi
Dep. Of Nutritional Sci. Fac. Of Applied Bioscience Tokyo Univ. Of Agriculture
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Arai Souichi
Department Of Nutritional Science Faculty Of Applied Bioscience Tokyo University Of Agriculture
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Mura K
Dep. Of Nutritional Sci. Fac. Of Applied Bioscience Tokyo Univ. Of Agriculture
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Mura Kiyoshi
Department Of Nutritional Science Tokyo University Of Agriculture
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KATO Saemi
Department of Nutritional Science, Faculty of Applied Bio-Science, Tokyo University of Agriculture
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Kato Saemi
Department Of Nutritional Science Faculty Of Applied Bio-science Tokyo University Of Agriculture
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SHIMIZU-IBUKA Akiko
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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Arai Soichi
Food Safety And Reliability Project Kanagawa Academy Of Science And Technology
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Arai Soichi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Shimizu Ibuka
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Mura Kiyoshi
Department Of Nutritional Science Faculty Of Applied Bioscience Tokyo University Of Agriculture
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ARAI Soichi
Department of Agricultural Chemicatry, The University of Tokyo
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