Molecular Cloning, Characterization, and Expression of Wheat Cystatins

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概要

• 論文の詳細を見る

• We cloned four kinds of cDNAs of wheat cystatins (WCs), WC1,WC2,WC3,and WC4,from the seed. They had 47-68% amino acid sequence similarities to other plant cystatins. WC1,WC2,and WC4 had 63-67% similalities to one another while 93% of amino acids were identical between WC1 and WC3. This suggested that WC1,WC2,and WC4 should be regarded as the isoforms of wheat cystatins. The mRNAs for WC1,WC2,and WC4 were all expressed in seed at an early stage of maturation and, after that, their quantities decreased gradually. However, each of the mRNAs was again expressed one day after the start of germination and the expression continued for the following five days. WC1 seemed to be expressed at a higher level than WC2 and WC4. Immunostaining for looking at sitespecific expression of each WC demonstrated that both WC1 and WC4 existed in the aleuron layer and embryo, but in the endosperm the only existing species was WC1. Differences in mRNA level and tissue localization found for the WCs may suggest their differential physiological roles.

• 社団法人日本農芸化学会の論文
• 2001-01-23

著者

• ARAI Soichi

  Department of Nutritional Science, Faculty of Applied Bioscience, Tokyo University of Agriculture

• Abe K

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• ABE Keiko

  Department of Pathology, Tohoku University Graduate School of Medicine

• Abe K

  Univ. Tokyo Tokyo Jpn

• Misaka Takumi

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Abe Keiko

  Agricultural And Life Sciences Dep. Of Applied Biological Chemistry

• Kuroda M

  Laboratory Of Medicinal Pharmacognosy Tokyo University Of Pharmacy And Life Sciences School Of Pharm

• Arai S

  Tokyo Univ. Agriculture Tokyo Jpn

• Arai S

  Graduate School Of Agricultural And Life Sciences The University Of Tokyo

• Arai S

  Department Of Applied Biological Chemistry The University Of Tokyo

• Arai Soichi

  Department Of Applied Biological Chemistry The University Of Tokyo

• Arai Soichi

  Department Of Applied Biological Chemistry Division Of Agriculture And Agricultural Life Sciences Th

• Arai Soichi

  Department Of Applied Biological Chemistry Division Of Agriculture And Agricultural Life Science The

• Arai Soichi

  Department Of Agricultural Chemistry The University Of Tokyo

• MATSUMOTO Ichiro

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U

• Abe K

  Univ Tokyo Tokyo Jpn

• Abe Keiko

  Department Of Cardiovascular Physiology And Medicine Graduate School Of Biomedical Sciences Hiroshim

• KURODA Masaharu

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U

• KIYOSAKI Toshihiro

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U

• Kiyosaki Toshihiro

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Arai Soichi

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Arai Soichi

  Department Of Nutrition Tokyo University Of Agriculture

• Abe K

  Faculty Of Pharmacy Musashino University

• Arai S

  Laboratory Of Environmental Biochemistry Graduate School Of Pharmaceutical Sciences Osaka University

• Ishimoto M

  National Agriculture Res. Center Ibaraki Jpn

• Kuroda M

  Laboratory Of Irrigation And Water Utilization Engineering Faculty Of Agriculture Kyushu University

• Kuroda Masaharu

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Koutaki Masahiro

  Biotron Institute Kyushu University

• Misaka Takumi

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Matsumoto I

  Univ. Tokyo Tokyo Jpn

• Arai Soichi

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Abe Keiko

  Department Of Agricultural Chemistry The University Of Tokyo

• Matsumoto Ichiro

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• ARAI Soichi

  Department of Agricultural Chemicatry, The University of Tokyo

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