Purification and Properties of a Cysteine Proteinase Occurring in Dormant Wheat Seeds
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概要
- 論文の詳細を見る
A cysteine proteinase was purified from dormant seeds of wheat (Triticum aestivum, cultivar Norin 61) and its molecular mass was estimated to be about 23kDa by gel filtration. The K_m of this proteinase at pH 5.5 was calculated as 2 μM for Z-Phe-Arg-MCA, and its activity was inhibited by cysteine proteinase inhibitors including antipain, E-64, and leupeptin. Oryzacystatin-I, a proteinaceous cysteine proteinase inhibitor in rice seeds, also inhibited its activity.
- 社団法人日本農芸化学会の論文
- 1997-04-23
著者
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ARAI Soichi
Department of Nutritional Science, Faculty of Applied Bioscience, Tokyo University of Agriculture
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Abe K
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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ABE Keiko
Department of Pathology, Tohoku University Graduate School of Medicine
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Abe K
Univ. Tokyo Tokyo Jpn
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Abe Keiko
Agricultural And Life Sciences Dep. Of Applied Biological Chemistry
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Kuroda M
Laboratory Of Medicinal Pharmacognosy Tokyo University Of Pharmacy And Life Sciences School Of Pharm
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Arai S
Tokyo Univ. Agriculture Tokyo Jpn
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Arai S
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Arai S
Department Of Applied Biological Chemistry The University Of Tokyo
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Arai Soichi
Department Of Applied Biological Chemistry The University Of Tokyo
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Arai Soichi
Department Of Applied Biological Chemistry Division Of Agriculture And Agricultural Life Sciences Th
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Arai Soichi
Department Of Applied Biological Chemistry Division Of Agriculture And Agricultural Life Science The
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Arai Soichi
Department Of Agricultural Chemistry The University Of Tokyo
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Abe K
Univ Tokyo Tokyo Jpn
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Abe Keiko
Department Of Cardiovascular Physiology And Medicine Graduate School Of Biomedical Sciences Hiroshim
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KURODA Masaharu
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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KIYOSAKI Toshihiro
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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Kiyosaki Toshihiro
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Arai Soichi
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Arai Soichi
Department Of Nutrition Tokyo University Of Agriculture
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Abe K
Faculty Of Pharmacy Musashino University
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Arai S
Laboratory Of Environmental Biochemistry Graduate School Of Pharmaceutical Sciences Osaka University
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Kuroda M
Laboratory Of Irrigation And Water Utilization Engineering Faculty Of Agriculture Kyushu University
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Kuroda Masaharu
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Koutaki Masahiro
Biotron Institute Kyushu University
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Arai Soichi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Abe Keiko
Department Of Agricultural Chemistry The University Of Tokyo
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Kiyosaki Toshihiko
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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ARAI Soichi
Department of Agricultural Chemicatry, The University of Tokyo
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