No Immunotoxic Effect on T Cells with Di(2-Ethylhexyl)Phthalate in Male C57BL/6 Mice

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Objectives: To clarify whether di (2-ethylhexyl) phthalate (DEHP) has immunotoxic effects on both the expression of surface molecules (CD3, CD4, CDS and CD28) on T cells of the thymus and spleen in male C57BL/6 mice. Methods: Animals were orally administered a 0.1% or 0.2% DEHP-containing diet for 10 or 20 days. Dietary corn oil was used as the vehicle for DEHP in preparing the diet. Results: Significant hepatic hypertrophy was clearly observed in the DEHP-exposed groups, while no atrophy was seen in the thymus or spleen in any treatment groups. In the thymus and spleen, no variation in the proportions of both T cells expressing CD3, CD4 and CDS was shown with cytometry analysis. The surface expression ofCD3, CD4, CDS and CD28 on both T cells was also invariable in all analyzed stages of thymic differentiation and in the spleen. No effect of DEHP on mitogenesis was shown in the splenic T cells with an in vitro [^3H]-thymidine-incorporation technique. Conclusions: DEHP is probably not an immunosuppressor, particularly for T cells.
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