Large-scale Production and Purification of an Erwinia ananas Ice Nucleation Protein and Evaluation of Its Ice Nucleation Activity

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概要

• 論文の詳細を見る

• The ice nucleation-active protein of Erwinia ananas IN-10 (inaA protein) was over-expressed as inclusion bodies in Escherichia coli in a yield of 15.3mg of inaA protein from 60mg of bacterial cells on a dry-matter basis. The inaA protein was purified from the inclusion bodies by solubilization with detergents to obtain a protein preparation free from sugar and lipid. This preparation had a distinct ice nucleation activity, indicating that the inaA protein per se is able to act as a nucleus.

• 社団法人日本農芸化学会の論文
• 1993-04-23

著者

• ARAI Soichi

  Department of Nutritional Science, Faculty of Applied Bioscience, Tokyo University of Agriculture

• Abe K

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• ABE Keiko

  Department of Pathology, Tohoku University Graduate School of Medicine

• Abe K

  Univ. Tokyo Tokyo Jpn

• Arai S

  Tokyo Univ. Agriculture Tokyo Jpn

• Arai S

  Department Of Applied Biological Chemistry The University Of Tokyo

• Arai Soichi

  Department Of Applied Biological Chemistry The University Of Tokyo

• Arai Soichi

  Department Of Applied Biological Chemistry Division Of Agriculture And Agricultural Life Sciences Th

• Arai Soichi

  Department Of Applied Biological Chemistry Division Of Agriculture And Agricultural Life Science The

• Arai Soichi

  Department Of Agricultural Chemistry The University Of Tokyo

• Abe Keiko

  Department Of Cardiovascular Physiology And Medicine Graduate School Of Biomedical Sciences Hiroshim

• WATANABE Michiko

  Faculty of Education, Tokyo Gakugei University

• Watanabe M

  Faculty Of Agriculture Tokyo University Of Agriculture And Technology

• Watabe Satoshi

  Department of Agricultural Chemistry, The University of Tokyo

• Hirata Aiko

  The Institute of Applied Microbiology, The University of Tokyo

• Emori Yasufumi

  Faculty of Science

• Arai Soichi

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Arai Soichi

  Department Of Nutrition Tokyo University Of Agriculture

• Watanabe M

  Department Of Health And Nutrition Takasaki University Of Health And Welfare

• Hirata Aiko

  The Institute Of Applied Microbiology The University Of Tokyo

• Watabe S

  Japan Tabacco Inc. Kanagawa Jpn

• Watabe S

  Laboratory Of Marine Biochemistry Faculty Of Agriculture University Of Tokyo

• Emori Yasufumi

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Emori Yasufumi

  Faculty Of Engineering Ibaragi University

• Watanabe M

  Tokyo Univ. Agriculture And Technology Tokyo Jpn

• Arai Soichi

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Watanabe Michiko

  Faculty Of Economics Toyo University

• Abe Keiko

  Department Of Agricultural Chemistry The University Of Tokyo

• ARAI Soichi

  Department of Agricultural Chemicatry, The University of Tokyo

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