Heterogeneous Nuclear Ribonucleoprotein K Interacts with and Is Proteolyzed by Calpain in vivo(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
Calpain is a cytosolic "modulator protease" that modulates cellular functions in response to Ca^<2+>. To identify in vivo substrates of calpain, yeast two-hybrid screening was done using the 5-EF-hand (penta-EF-hand ; PEF) domain of the μ-calpain large subunit (domain IV), since several possible in vivo substrates for calpain have been previously reported to bind to the 5-EF-hand domains. Other than the regulatory subunit of calpain, which binds to the domain IV, heterogeneous nuclear ribonucleoproteins (hnRNP) K and R were identified, and shown to be proteolyzed by μ-calpain in vitro. When expressed in COS7 cells, hnRNP K and μ-calpain co-localized in the cytosol, and Ca^<2+>-ionophore stimulation of the cells resulted in proteolysis of hnRNP K, indicating that hnRNP K is an in vivo substrate for calpain. Now, hnRNP K is considered to function as a scaffold protein for its binding proteins, such as PKCδ and C/EBPβ, which were reported to be calpain substrates, suggesting that hnRNP-K is a scaffold for calpain to proteolyze these proteins.
- 社団法人日本農芸化学会の論文
- 2003-08-23
著者
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ABE Keiko
Department of Pathology, Tohoku University Graduate School of Medicine
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Abe Kohji
Laboratory Of Nutrition Chemistry
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Suzuki Kazuharu
Department Of Nutritional Science Tokyo University Of Agriculture
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Suzuki Kazuharu
東京農業大学 農学部栄養科学科
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Abe K
Agricultural And Life Sciences Dep. Of Applied Biological Chemistry
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Abe Keiko
Agricultural And Life Sciences Dep. Of Applied Biological Chemistry
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KIMURA EIICHI
Department of Internal Medicine. Nippon Medical School
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Abe Keiko
Department Of Cardiovascular Physiology And Medicine Graduate School Of Biomedical Sciences Hiroshim
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Sorimachi Hiroyuki
Department Of Enzymatic Regulation For Cell Functions Tokyo Metropolitan Institute Of Medical Scienc
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Suzuki K
Univ. Tokyo Tokyo
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Suzuki K
Taisho Pharmaceutical Co. Ltd. Saitama Jpn
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Suzuki K
Department Of Nutritional Science Tokyo University Of Agriculture
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SUZUKI Koichi
Tokyo Metropolitan Institute of Gerontology
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KIMURA Eiichi
Institute of Pharmaceutical Sciences Hiroshima University School of Medicine
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Abe K
Laboratory Of Enzymology Graduate School Of Agricultural Science Tohoku University
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Kimura E
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Abe Keietsu
Divison Of Applied Life Science Graduate School Of Agricultural Science Tohoku University
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Kimura Eiichi
Department Of Critical Care Med Nippon Medical School
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Kimura Eiichi
Department Of Bioengineering Tokyo Institute Of Technology
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Abe Keiko
Department Of Agricultural Chemistry The University Of Tokyo
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Sorimachi Hiroyuki
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo:CREST, JST
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Kimura Eiichi
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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SORIMACHI Hiroyuki
Department of Advanced Science for Biomolecules, Tokyo Metropolitan Institute of Medical Science
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