Cloning and Expression of an Endo-1,6-β-D-glucanase Gene (neg1) from Neurospora crassa(Biochemistry & Molecular Biology)

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概要

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• A gene (neg1) encoding an endo-1,6-β-D-glucanase from Neurospora crassa was cloned. The putative neg1 was 1443-bp long and encoded a mature endo-1,6-β-D-glucanase protein of 463 amino acids and signal peptide of 17 amino acids. The purified recombinant protein (Neg1) obtained from Escherichia coli showed 1,6-β-D-glucanase activity. No genes similar in sequence were found in yeasts and fungl.

• 社団法人日本農芸化学会の論文
• 2002-06-23

著者

• Abe Kohji

  Laboratory Of Nutrition Chemistry

• Abe K

  Agricultural And Life Sciences Dep. Of Applied Biological Chemistry

• NAKAJIMA Tasuku

  Laboratory of Enzymology, Department of Molecular and Cell Biology, Graduate School of Agricultural

• ABE Keietsu

  Laboratory of Enzymology, Graduate School of Agriculture, Tohoku University

• YAMAGATA Youhei

  Department of Agriscience and Bioscience, Tokyo University of Agriculture and Technology

• Nakajima T

  Laboratory Of Enzymology Graduate School Of Agricultural Science Tohoku University

• YAMAGATA Youhei

  Laboratory of Enzymology, Department of Molecular and Cell Biology, Graduate School of Agricultural

• Abe K

  Laboratory Of Enzymology Graduate School Of Agricultural Science Tohoku University

• OYAMA Seiji

  Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University

• Nakajima Tasuku

  Laboratory Of Enzymology Division Of Life Science Graduate School Of Agricultural Science Tohoku Uni

• Oyama Seiji

  Laboratory Of Enzymology Graduate School Of Agricultural Science Tohoku University

• Yamagata Yukari

  Laboratory Of Enzymology Graduate School Of Agricultural Science Tohoku University

• Yamagata Y

  Tohoku Univ. Sendai Jpn

• Abe Keietsu

  Divison Of Applied Life Science Graduate School Of Agricultural Science Tohoku University

• Abe Keietsu

  Laboratory Of Enzymology Department Of Molecular And Cell Biology Graduate School Of Agricultural Sc

• Yamagata Youhei

  Dep. Of Applied Molecular Biology And Biochemistry Tokyo Univ. Of Agriculture And Technol.

• Yamagata Youhei

  Department Of Applied Molecular Biology And Biochemistry Tokyo University Of Agriculture And Technol

• Yamagata Youhei

  Laboratory Of Enzymology Department Of Molecular And Cell Biology Graduate School Of Agricultural Sc

• Nakajima Tasuku

  Laboratory Of Enzymology Department Of Molecular And Cell Biology Graduate School Of Agricultural Sc

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