The SskA and SrrA Response Regulators Are Implicated in Oxidative Stress Responses of Hyphae and Asexual Spores in the Phosphorelay Signaling Network of Aspergillus nidulans
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概要
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Histidine-to-Aspartate (His-Asp) phosphorelay (or two-component) systems are common signal transduction mechanisms implicated in a wide variety of cellular responses to environmental stimuli in both prokaryotes and eukaryotes. For a model filamentous fungi, Aspergillus nidulans, in this study we first compiled a complete list of His-Asp phosphorelay components, including 15 genes for His-kinase (HK), four genes for response regulator (RR), and only one for histidine-containing phosphotransfer intermediate (HPt). For these RR genes, a set of deletion mutants was constructed so as to create a null allele for each. When examined these mutant strains under various conditions stressful for hyphal growth and asexual spore development, two of them (designated ΔsskA and ΔsrrA) showed a marked phenotype of hypersensitivity to oxidative stresses (particularly, to hydrogen peroxide). In this respect, expression of the vegetative-stage specific catB catalase gene was severely impaired in both mutants. Furthermore, conidia from ΔsskA were hypersensitive not only to treatment with H2O2, but also to treatment at aberrantly low (4 °C) and high (50 °C) temperatures, resulting in reduced germination efficiency. In this respect, not only the catA catalase gene specific for asexual development, but also a set of genes encoding the enzymes for synthesis of certain stress tolerant compatible solutes, such as trehalose and glycerol, were markedly downregulated in conidia from ΔsskA. These results together are indicative of the physiological importance of the His-Asp phosphorelay signaling network involving the SskA and SrrA response regulators.
- 社団法人 日本農芸化学会の論文
著者
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Kato Masashi
Laboratory of Gene Regulation, Graduate School of Bioagricultural Sciences, Nagoya University
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Kobayashi Tetsuo
Laboratory of Gene Regulation, Graduate School of Bioagricultural Sciences, Nagoya University
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YAMASHINO Takafumi
Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University
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MIZUNO Takeshi
Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University
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Kanamaru Kyoko
Laboratory of Gene Regulation, Graduate School of Bioagricultural Sciences, Nagoya University
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Hagiwara Daisuke
Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University
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Asano Yoshihiro
Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University
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Marui Junichiro
Laboratory of Gene Regulation, Graduate School of Bioagricultural Sciences, Nagoya University
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FURUKAWA Kentaro
Laboratory of Enzymology, Graduate School of Agriculture, Tohoku University
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ABE Keietsu
Laboratory of Enzymology, Graduate School of Agriculture, Tohoku University
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