Cloning, Nucleotide Sequence, and Hyperexpression of α-Amylase Gene from an Archaeon, Thermococcus profundus

スポンサーリンク

概要

• 論文の詳細を見る

• A thermostable α-amylase gene of a novel hyperthermophilic archaeon, Thermococcus profundus, was cloned and expressed in Escherichia coli. E. coli cells transformed with the gene overproduced the amylase to a level as much as 155.5-fold higher than that produced by T. profundus. Up to 45% of the total activity was secreted into periplasmic fraction and culture filtrate. The nucleotide sequence of the gene displayed an open reading frame of 1,203 bp encoding a 401-amino acid protein, which was composed of a 26-amino acid signal peptide and a 375-amino acid mature enzyme. Analysis of the deduced amino acid sequence revealed that four homologous regions common to amylases were conserved in the α-amylase. The molecular mass of the mature enzyme was calculated to be 43,281 Da, which is consistent with the value obtained from results of SDS-poly-acrylamide gel electrophoresis of the purified enzyme. The amylase purified to homogeneity from the E. coli transformant exhibited properties identical to those of native amylase from T. profundus in terms of thermoactivity, pH profile, and the mode of action on various substrates. The amylase did not hydrolyze α-1,6-glucosidic linkages.

• 社団法人日本生物工学会の論文
• 1996-11-25

著者

• Kobayashi Tetsuo

  Laboratory of Gene Regulation, Graduate School of Bioagricultural Sciences, Nagoya University

• Kudo Toshiaki

  Environmental Molecular Biology Lab. :graduate School Of Integrated Science Yokohama City University

• Kudo Toshiaki

  Laboratory Of Microbiology The Institute Of Physical And Chemical Research(riken)

• KANAI HARUHIKO

  Laboratory of Microbiology, the Institute of Physical and Chemical Research(RIKEN)

• LEE JONG

  Laboratory of Microbiology, The Institute of Physical and Chemical Research(Present address)Korea Gi

• AKIBA TERUHIKO

  Laboratory of Microbiology, The Institute of Physical and Chemical Research

• Takio K

  Enviromental Molecular Biology Lab. Riken

• Kudo T

  Inst. Physical And Chemical Res.(riken) Saitama Jpn

• Kudo T

  Riken Saitama Jpn

• Kudo Toshiaki

  International Graduate School Of Arts And Sci. Yokohama City Univ.

• Kudo Toshiaki

  Yokohama City Univ. Kanagawa Jpn

• Lee Jong

  Laboratory Of Applied Entomology Faculty Of Agriculture Yamaguchi University

• Kobayashi Tetsuo

  Dep. Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya Univ.

• Kudo Toshiaki

  Laboratory Of Microbiology The Institute Of Physical And Chemical Research

• Akiba Teruhiko

  Laboratory Of Microbiology The Institute Of Physical And Chemical Research

• Kanai Haruhiko

  Laboratory Of Microbiology The Institute Of Physical And Chemical Research (riken)

• Kobayashi T

  Laboratory Of Microbiology The Institute Of Physical And Chemical Research (riken)

• Kobayashi T

  Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya

• Kobayashi Tetsuo

  Laboratory Of Gene Regulation Graduate School Of Bioagricultural Sciences Nagoya University

• Lee Jong

  Laboratory Of Microbiology The Institute Of Physical And Chemical Research(present Address)korea Gin

• Kobayashi Tetsuo

  Laboratory of Agricultural Meteorology, Faculty of Agriculture, Kyushu University

関連論文

• High-throughput screening of DNA binding sites for transcription factor AmyR from Aspergillus nidulans using DNA beads display system(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Functional Expression of Three Rieske Non-Heme Iron Oxygenases Derived from Actinomycetes in Rhodococcus Species for Investigation of Their Degradation Capabilities of Dibenzofuran and Chlorinated Dioxins
• Expression of a Novel 90-kDa Protein, Lsd90, Involved in the Metabolism of Very Long-chain Fatty Acid-containing Phospholipids in a Mitosis-defective Fission Yeast Mutant
• PA-42 An alternative pathway of primary metabolism of termite gut protists inferred from meta-EST analysis(Symbiosis/Cell to cell interaction,Poster presentation A)
• Effect of Temporal Sample Preservation on the Molecular Study of a Complex Microbial Community in the Gut of the Termite Microcerotermes sp
• Identification and in situ Detection of Two Lineages of Bacteroidales Ectosymbionts Associated with a Termite Gut Protist, Oxymonas sp.
• Fungal Community Analysis of Fungus Gardens in Termite Nests
• Isolation and Detection of Methanogens from the Gut of Higher Termites
• Gut of Higher Termites as a Niche for Alkaliphiles as Shown by Culture-Based and Culture-Independent Studies
• Nitrogen Fixation Genes Expressed in the Symbiotic Microbial Community in the Gut of the Termite Coptotermes formosanus
• Molecular Phylogeny of Symbiotic Basidiomycetes of Fungus-growing Termites in Thailand and Their Relationship with the Host(Microbiology & Fermentation Technology)
• Diverse Bacteria Related to the Bacteroides Subgroup of the CFB Phylum within the Gut Symbiotic Communities of Various Termites(Microbiology & Fermentation Technology)
• Purification and Characterization of L-2,3-Butanediol Dehydrogenase of Brevibacterium saccharolyticum C-1012 Expressed in Escherichia coli(Microbiology & Fermentation Technology)
• Characterization of the NADH-Linked Acetylacetoin Reductase/2, 3-Butanediol Dehydrogenase Gene from Bacillus cereus YUF-4
• Cloning, Expression and Nucleotide Sequence of the L-2, 3-Butanediol Dehydrogenase Gene from Brevibacterium saccharolyticum C-1012
• Molecular Generation of an Escheichia coli Srain Pproducing Only the meso-Isomer of 2,3-Butanediol
• Sequence Analysis of the Gene for and Characterization of D-Acetoin Forming meso-2,3-Butanediol Dehydrogenase of Klebsiella pneumoniae Expressed in Escherichia coli
• Cloning, Nucleotide Sequence, and Hyperexpression of α-Amylase Gene from an Archaeon, Thermococcus profundus
• Preparation of a Chiral Acetoinic Compound Using Transgenic Escherichia coli Expressing the 2,3-Butanediol Dehydrogenase Gene
• An Alkaliphilic and Xylanolytic Paenibacillus Species Isolated from the Gut of a Soil-Feeding Termite
• A shaE Deletion Mutant Showed Lower Na^+ Sensitivity Compared to Other Deletion Mutants in the Bacillus subtilis Sodium/Hydrogen Antiporter (Sha) System
• Molecular ecology of termite-microorganisms symbiotic systems(Symposium on Various Interactions between Animal and Microorganism)
• Production, and Biological Characterization, of Dideoxygriseorhodin C by a Streptomyces sp. and Its Taxonomy
• Nucleotide Sequence of the Xylanase A Gene of Alkalophilic Bacillus sp. Strain C-125(Microbiology & Fermentation Industry)
• Extracellular Production of Alkaline Xylanase of Alkalophilic Bacillus sp. by Escherichia coli Carrying pCX311
• Extracellular Production of Xylanase L in Escherichia coli using Excretion Vector pEAP2
• Purification and Partial Characterization of Alkaline Xylanase from Escherichia coli Carrying pCX311
• Molecular Cloning and Expression of β-Isopropylmalate Dehydrogenase Gene from Alkalophilic Bacillus in Escherichia coli
• Isolation of the Fragments from Alkalophilic Bacillus sp. DNA Which are Controlled by Temperature, pH or NaCl Concentration in pGR71 CAT Expression System
• Gene Expression and Production of Bacillus No. 170 Penicillinase in Escherichia coli and Bacillus subtilis
• Molecular Cloning of β-isopropylmalate dehydrogenase Gene from Clostridium butyricum M588
• The Effect of pH on Heat-resistance of Spores of Alkalophilic Bacillus No. 2b-2
• Effect of pH and Sodium Ion on Germination of Alkalophilic Bacillus Species
• Plasmids in Alkalophilic Bacillus sp.
• Molecular Cloning, Nucleotide Sequence and Expression in Escherichia coli of Hyperthermophilic Glutamate Dehydrogenase Gene from Thermococcus profundus
• Properties of Glutamate Dehydrogenase and Its Involvement in Alanine Production in a Hyperthermophilic Archaeon, Thermococcus Profundus^1
• Location of F_1 ATPase-like Genes on the Physical Map of the Bacillus subtilis 168 Chromosome
• Crystal Structure of meso-2,3-Butanediol Dehydrogenase in a Complex with NAD^+ and Inhibitor Mercaptoethanol at 1.7 A Resolution for Understanding of Chiral Substrate Recognition Mechanisms
• Carbon mineralization by termites in tropical forests, with emphasis on fungus combs
• The SskA and SrrA Response Regulators Are Implicated in Oxidative Stress Responses of Hyphae and Asexual Spores in the Phosphorelay Signaling Network of Aspergillus nidulans
• Novel Reporter Gene Expression Systems for Monitoring Activation of the Aspergillus nidulans HOG Pathway
• Isolation and Characterization of Dibenzofuran-degrading Actinomycetes : Analysis of Multiple Extradiol Dioxygenase Genes in Dibenzofuran-degrading Rhodococcus Species(Biochemistry & Molecular Biology)
• P-405 SITE-SPECIFIC FUNCTIONALIZATION OF PROTEINS BY ORGANOPALLADIUM REACTIONS
• Physiological Properties of a Neutralo-sensitive Mutant Derived from Facultative Alkaliphilic Bacillus sp. C-125
• Characterization of a Mutation Responsible for an Alkali-sensitive Mutant, 18224, of Alkaliphilic Bacillus sp. strain C-125
• Characteristics of Alkali-sensitive Mutants of Alkaliphilic Bacillus sp. Strain C-125 That Show Cellular Morphological Abnormalities
• Phylogenetic and Morphological Diversity of Bacteroidales Members Associated with the Gut Wall of Termites
• Expression Profile of Amylolytic Genes in Aspergillus nidulans
• The Region in a Subunit of the Aspergillus CCAAT-Binding Protein Similar to the HAP4p-Recruiting Domain of Saccharomyces cerevisiae Hap5p Is Not Essential for Transcriptional Enhancement
• Mode of AmyR Binding to the CGGN_8AGG Sequence in the Aspergillus oryzae taaG2 Promoter
• No Factors Except for the Hap Complex increase the Taka-amylase A Gene Expression by Binding to the CCAAT Sequence in the Promoter Region(Biochemistry & Molecular Biology)
• In Vivo and in Vitro Analyses of the AmyR Binding Site of the Aspergillus nidulans agdA Promoter ; Requirement of the CGG Direct Repeat for Induction and High Affinity Binding of AmyR(Biochemistry & Molecular Biology)
• A Simple and Rapid Method for the Preparation of a Cell-free Extract with CCAAT-Binding Activity from Filamentous Fungi
• Two Sets of Biphenyl and PCB Degradation Genes on a Linear Plasmid in Rhodococcus erythropolis TA421
• A Gram-positive Polychlorinated Biphenyl-degrading Bacterium, Rhodococcus eryth-ropolis Strain TA421, Isolated from a Termite Ecosystem
• Enzymatic Hydrolysis of Fats at High Substrate Concentrations in Biphasic Organic-Aqueous Systems
• Kinetics of Enzymatic Hydrolysis of Lipids in Biphasic Organic-Aqueous Systems
• Purification and Characterization of α-Amylase from Hyperthermophilic Archaeon Thermococcus profundus, Which Hydrolyzes Both α-1, 4 and α-1, 6 Glucosidic Linkages
• Extracellular Production of Human Immunoglobulin ε-Chain/γ1-Chain Chimeric Fc Polypeptide by Escherichia coli
• Extracellular Production of Human Tumor Necrosis Factor-α by Escherichia coli Using a Chemically-synthesized Gene(Biological Chemistry)
• Cloning and Characterization of the Azurin iso-1 Gene, Concerned with the Electron Transport Chain Involved in Methylamine/Methanol Oxidation in the Obligate Methylotroph Methylomonas sp. Strain J
• Genetic Organization and Characterization of the mau Gene Cluster, Which Concerned the Initial Step of Electron Transport Chains Involved in Methylamine Oxidation of the Obligate Methylotroph Methylomonas sp. Strain J
• Physiological Properties of Two Pseudomonas mendocina Strains Which Assimilate Styrene in a Two-Phase (Solvent-Aqueous) System under Static Culture Conditions
• Production of 3-Vinylcatechol and Physiological Properties of Pseudomonas LF-3, Which Can Assimilate Styrene in a Two-phase (Solvent-Aqueous) System
• IMMATURE STAGES OF PYRRHALTA HUMERALIS (CHEN) AND GALERUCA VICINA SOLSKY FROM JAPAN (COLEOPTERA, CHRYSOMELIDAE)
• Morphological Studies on the Immature Stages of Two Japanese Species of the Genus Galerucella (Coleoptera, Chrysomelidae)
• A Comparison Experiment of Two Types of Printed Circuit Board TDR Probes
• GFP-Tagged Expression Analysis Revealed That Some Histidine Kinases of Aspergillus nidulans Show Temporally and Spatially Different Expression during the Life Cycle
• In Vitro Analysis of His-Asp Phosphorelays in Aspergillus nidulans : The First Direct Biochemical Evidence for the Existence of His-Asp Phosphotransfer Systems in Filamentous Fungi
• The SskA and SrrA Response Regulators Are Implicated in Oxidative Stress Responses of Hyphae and Asexual Spores in the Phosphorelay Signaling Network of Aspergillus nidulans
• Characterization of the NikA Histidine Kinase Implicated in the Phosphorelay Signal Transduction of Aspergillus nidulans, with Special Reference to Fungicide Responses
• Genomics of Aspergillus oryzae
• Inducer-Dependent Nuclear Localization of a Zn(II)_2Cys_6 Transcriptional Activator, AmyR, in Aspergillus nidulans
• Functional Expression of Three Rieske Non-Heme Iron Oxygenases Derived from Actinomycetes in Rhodococcus Species for Investigation of Their Degradation Capabilities of Dibenzofuran and Chlorinated Dioxins
• Novel Promoter Sequence Required for Inductive Expression of the Aspergillus nidulans Endoglucanase Gene eglA
• Novel Reporter Gene Expression Systems for Monitoring Activation of the Aspergillus nidulans HOG Pathway
• Site-specific incorporation of 4-lodo-L-phenylalanine through opal suppression
• A Hyperthermophilic Sulfur-reducing Archaebacterium, Thermococcus sp. DT1331,Isolated from a Deep-sea Hydrothermal Vent
• Cloning, Expression, and Characterization of a Minor Alkaline Protease from Bacillus sp. No. AH-101
• Substrate Specificity of Thermostable Alkaline Protease from Bacillus sp. No. AH-101
• Polychlorinated Biphenyl/Biphenyl Degrading Gene Clusters in Rhodococcus sp. K37, HA99, and TA431 Are Different from Well-Known bph Gene Clusters of Rhodococci
• Xylose Triggers Reversible Phosphorylation of XlnR, the Fungal Transcriptional Activator of Xylanolytic and Cellulolytic Genes in aspergillus oryzae
• Fluorometric Determination of Diarrhetic Shellfish Toxins by High-Performance Liquid Chromatography(Analytical Chemistry)
• Chronopsies Assess the Within-day Variability of Blood Pressure and Pulse
• A Method for Analyzing Local Climates by Using an Infrared Radiation Thermometer together with Topographic Factors
• Comprehensive Analysis of the DNA-Binding Specificity of an Aspergillus nidulans Transcription Factor, AmyR, Using a Bead Display System

もっと見る 閉じる

スポンサーリンク