In Vitro Analysis of His-Asp Phosphorelays in Aspergillus nidulans : The First Direct Biochemical Evidence for the Existence of His-Asp Phosphotransfer Systems in Filamentous Fungi

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概要

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• His-Asp phosphorelays are widespread signal transduction mechanisms in bacteria, fungi, and higher plants. In order to investigate a His-Asp phosphorelay network in filamentous fungi, which has been genetically characterized in part, we attempted to construct an in vitro phosphotransfer network in Aspergillus nidulans comprising all the necessary components. As a first step, we established an in vitro phosphotransfer system with a histidine-containing phosphotransmitter YpdA, a response regulator SrrA, and a bacterial histidine kinase ArcB as a phosphate donor. We demonstrated the phosphotransfer from ArcB to A. nidulans YpdA and the subsequent transfer from YpdA to SrrA. This is the first direct biochemical evidence for the presence of the phosphotransfer system in filamentous fungi. Furthermore, a retrograde phosphorylation from YpdA to FphA, a histidine kinase similar to bacterial phytochrome, was found. The overall picture of the His-Asp phosphorelays in A. nidulans is discussed based on the results of the in vitro study.

• 社団法人 日本農芸化学会の論文
• 2007-10-23

著者

• Kato Masashi

  Laboratory of Gene Regulation, Graduate School of Bioagricultural Sciences, Nagoya University

• Kobayashi Tetsuo

  Laboratory of Gene Regulation, Graduate School of Bioagricultural Sciences, Nagoya University

• YAMASHINO Takafumi

  Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University

• MIZUNO Takeshi

  Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University

• MATSUSHIKA Akinori

  Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University

• Yamashino Takafumi

  Nagoya Univ. Nagoya Jpn

• Mizuno Takeshi

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• Yamashino T

  Nagoya Univ. Nagoya Jpn

• Mizuno Takeshi

  Lab. Of Molecular Microbiology School Of Agriculture Nagoya Univ.

• Mizuno T

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• Kanamaru Kyoko

  Laboratory of Gene Regulation, Graduate School of Bioagricultural Sciences, Nagoya University

• Ashikari M

  Bioscience And Biotechnology Center Nagoya University

• Ashikari Motoyuki

  Bio-oriented Technology Research Advancement Institution:(present Address)bioscience Center Nagoya U

• Matsushika Akinori

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• Kato Masashi

  Laboratory Of Gene Regulation Graduate School Of Bioagricultural Sciences Nagoya University

• Ashikari Motoyuki

  Bioscience And Biotechnology Center Nagoya Univ.

• Yamashino Takafumi

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• Moritz Thomas

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• AZUMA Nobuhiro

  Laboratory of Gene Regulation, Graduate School of Bioagricultural Sciences, Nagoya University

• Azuma Nobuhiro

  Laboratory Of Gene Regulation Graduate School Of Bioagricultural Sciences Nagoya University

• Kobayashi Tetsuo

  Laboratory Of Gene Regulation Graduate School Of Bioagricultural Sciences Nagoya University

• Kato Masashi

  Dep. Of Material And Environmental Chemistry Graduate School Of Engineering Utsunomiya Univ.

• Kanamaru Kyoko

  Lab. Of Gene Regulation Graduate School Of Bioagricultural Sciences Nagoya Univ.

• Kanamaru Kyoko

  Laboratory Of Gene Regulation Graduate School Of Bioagricultural Sciences Nagoya University

• Mizuno Takeshi

  Laboratory Of Microbiology School Of Agriculture Nagoya University

• Kobayashi Tetsuo

  Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya

• Mizuno T

  Faculty Of Agriculture Tokyo University Of Agriculture And Technology

• YAMASHINO Takafumi

  Laboratory of Molecular and Functional Cenomics, School of Agriculture, Nagoya University

• Kobayashi Tetsuo

  Laboratory of Agricultural Meteorology, Faculty of Agriculture, Kyushu University

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