Deletion of the yhhP Gene Results in Filamentous Cell Morphology in Escherichia coil

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概要

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• The Escherichia coli yhhP gene was predicted to encode a small hypothetical protein of 81 amino acids, the cellular function of which is not known. To gain insight into the function of this uncharacterized YhhP protein, genetic and biochemical studies were done. We first tried to express and purify the YhhP protein to prepare an anti-YhhP antiserum. Western blotting showed that the hypothetical yhhP gene is indeed transcribed and translated as a minor cytoplasmic protein. YhhP-deficient (Δ yhhP) cells formed colonies poorly on a rich medium (e.g., Luria-Bertani medium) containing a relatively low concentration of NaCl, while they can grow normally either in LB containing 3% NaCl or in a synthetic medium (e.g., M9-glucose). During exponential growth in rich medium, an early step of cell division was inhibited in Δ yhhP cells, forming filaments. For the YhhP-deficient filamentous cells, the FtsZ-ring formation was analyzed with immunofluorescence microscopy. The FtsZ-ring formation did not occur normally in the Δ yhhP filaments, although the filamentous cells contained the FtsZ protein at a certain level comparable to that in the wild-type cells. The ftsZ gene was found to function as a multicopy suppressor of the Δ yhhP mutant. Another multicopy suppressor gene was identified as the dksA gene. Provided that either the ftsZ or dksA gene was introduced into the mutant cells with its multicopy state, the resulting transformants were capable of growing in rich medium, formed wild-type short rods. These results are discussed with regard to the presumed function of this ubiquitous protein.

• 社団法人日本農芸化学会の論文
• 2000-04-23

著者

• Yamada Hideyuki

  SEIREN CO. LTD.

• YAMASHINO Takafumi

  Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University

• MIZUNO Takeshi

  Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University

• YAMADA Hisami

  Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University

• OHASHI Kenji

  Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University

• Mizuno Takeshi

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• Ishii Yuko

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• Yamazaki Toshimasa

  Structural Biology Unit National Institute Of Agrobiological Resources

• Yamada Hidenori

  Department Of Bioengineering Science Faculty Of Engineering Okayama University

• Yamada Hisami

  Laboratory Of Microbiology School Of Agriculture Nagoya University

• Katoh E

  National Inst. Agrobiological Resources Tsukuba Jpn

• Katoh Etsuko

  Structural Biology Unit National Institute Of Agrobiological Resources

• Yamada Hisami

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• SHINDO Heisaburo

  Tokyo University of Pharmacy and Life Science

• Yamashino Takafumi

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• Ohashi Kenji

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• Mizuno Takeshi

  Laboratory Of Microbiology School Of Agriculture Nagoya University

• YAMASHINO Takafumi

  Laboratory of Molecular and Functional Cenomics, School of Agriculture, Nagoya University

• Shindo Heisaburo

  Tokyo College of Pharmacy

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