Construction and Characterization of a Deletion Mutant of gpd2 That Encodes an Isozyme of NADH-Dependent Glycerol-3-phosphate Dehydrogenase in Fission Yeast

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概要

• 論文の詳細を見る

• Schizosaccharomyces pombe has two genes each encoding an isozyme of NADH-dependent glycerol-3-phosphate dehydrogenases (gpd1^+ and gpd2^+). To gain an insight into the function of these genes, here we constructed a gpd2 deletion mutant, in addition to the previously constructed gpd1 deletion mutant. We showed that the gpd1^+ and gpd2^+ gene-products are both funcional in terms of the de novo glycerol synthesis. Furthermore, the gpd1^+-mediated glycerol production is primarily responsible for the osmoregulation, but the gpd2^+ gene is not. Interestingly, however, the gpd2 deletion mutant had histidine- or lysine-auxotrophy for growth on a minimal medium.

• 社団法人日本農芸化学会の論文
• 1996-05-23

著者

• Ohmiya Ryusuke

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• Yamada Hideyuki

  SEIREN CO. LTD.

• MIZUNO Takeshi

  Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University

• YAMADA Hisami

  Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University

• AIBA Hirofumi

  Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University

• Mizuno Takeshi

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• Aiba H

  Nagoya Univ. Nagoya Jpn

• Aiba Hirofumi

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• Aiba Hirofumi

  名古屋大学 農学部微生物学

• Aiba Hirofumi

  Laboratory Of Microbiology Faculty Of Agriculture Nagoya University

• Ohmiya R

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• Yamada Hidenori

  Department Of Bioengineering Science Faculty Of Engineering Okayama University

• Aiba H

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• Yamada Hisami

  Laboratory Of Microbiology School Of Agriculture Nagoya University

• Mizuno Takeshi

  Laboratory Of Microbiology School Of Agriculture Nagoya University

• Aiba Hirofumi

  Laboratory Of Molecular Microbiology Graduate School Of Bioagricultural Sciences Nagoya University

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