A Novel Gene That Interferes with the Phosphotransfer Signal Transduction Mediated by the EnvZ Osmosensor in Escherichia coli
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概要
- 論文の詳細を見る
In Escherichia coli, expression of the major outer membrane proteins, OmpC and OmpF, is regulated in response to the medium osmolarity and other environmental stimuli. A two-component signal transduction system, mediated by EnvZ and OmpR, is crucially responsible for this osmotic regulation of the ompC and ompF genes. In this study, an E. coli gene was cloned, which interferes with expression of both the ompC and ompF genes at the level of transcription, provided that the cloned gene was introduced in E. coli cells by a multicopy plasmid. The gene product was identified as F107, which was previously characterized as a hypothetical protein in E. coli genome databases. F107 containing 107 amino acids appears to be highly hydrophobic, and has a sequence similarity to the eukaryotic type of cytochrome-c oxidase subunit III. The mechanism by which F107 inhibits transcription of ompC and ompF was examined extensively, mainly by using a set of envZ and ompR mutants. These results suggested that F107 interferes specifically with a function of the EnvZ osmosensory kinase. Possible mechanisms by which F107 affect the EnvZ function are discussed.
- 社団法人日本農芸化学会の論文
- 1996-10-23
著者
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MIZUNO Takeshi
Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University
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AIBA Hirofumi
Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University
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Mizuno Takeshi
Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University
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Aiba H
Nagoya Univ. Nagoya Jpn
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Aiba Hirofumi
Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University
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Aiba Hirofumi
名古屋大学 農学部微生物学
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Aiba Hirofumi
Laboratory Of Microbiology Faculty Of Agriculture Nagoya University
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HIROKAWA Kozo
Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University
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OGINO Tomoaki
Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University
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Ogino Tomoaki
Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University
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Aiba H
Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University
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Hirokawa K
Kikkoman Corp. Chiba Jpn
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Mizuno Takeshi
Laboratory Of Microbiology School Of Agriculture Nagoya University
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Aiba Hirofumi
Laboratory Of Molecular Microbiology Graduate School Of Bioagricultural Sciences Nagoya University
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