A Rapid Method for Disrupting Genes in the Escherichia coli Genome

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概要

• 論文の詳細を見る

• The entire genomic sequence of Escherichia coli has recently been completed. To gain insight into the function of the vast array of yet uncharacterized open reading frames (ORFs), a variety of new genetic tools will be required. Here we examined a genetic system, using an integration plasmid vector (named pINT007), for rapid construction of disruption mutants of any ORF in E. coli. It was found that the vector allows us to rapidly construct a disruption mutant for any gene on the chromosome as a cointegrate, furthermore, resolution of the resulting cointegrate can be surely accomplished by using a pair of the bla (ampicillin resistant) genes on the vector as a positive-selection marker.

• 社団法人日本農芸化学会の論文
• 1998-09-23

著者

• Ohmiya Ryusuke

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• MIZUNO Takeshi

  Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University

• KATO Chinatsu

  Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University

• Kato C

  Japan Marine Sci. And Technol. Kanagawa Jpn

• Kato Chinatsu

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• Mizuno Takeshi

  Laboratory Of Microbiology School Of Agriculture Nagoya University

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