Rapid Response of Arabidopsis T87 Cultured Cells to Cytokinin through His-to-Asp Phosphorelay Signal Transduction

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概要

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• According to the current consistent model for the higher plant Arabidopsis thaliana, the scheme for an immediate early response to the plant hormone cytokinin can be formulated as Arabidopsis histidine kinase (AHK) cytokinin receptor-mediated His → Asp phosphorelay signal transduction. Nonetheless, clarification of the comprehensive picture of cytokinin-mediated signal transduction in this higher plant is at a very early stage. As a new approach to this end, we studied whether or not a certain Arabidopsis cell line (named T87) would be versatile for such work on cytokinin signal transduction. We show that T87 cells had the ability to respond to cytokinin, displaying the immediate early induction of type-A Arabidopsis response regulator (ARR) family genes (e.g., ARR6) at the transcriptional level. This event was further confirmed by employing the stable transgenic lines of T87 cells with a set of ARR::LUC reporter transgenes. We also show that T87 cells had the ability to respond to auxin when the expression of a set of AUX/IAA genes (e.g., IAA5) was examined. As postulated for intact plants, in T87 cells too, the induction of IAA5 by auxin was selectively inhibited in the presence of a proteasome inhibitor, while the induction of ARR6 by cytokinin was not significantly affected under the same conditions. Through transient expression assays with T87 protoplasts, it is shown that the intracellular localization profiles of the phosphorelay intermediate Arabidopsis histidine-containing phosphotransfer factor (AHPs; e.g., AHP1 and AHP4) were markedly affected in response to cytokinin, but those of type-A ARRs were not (e.g., ARR15 and ARR16). Taken together, we conclude that, in T87 cells, the AHK-dependent His → Asp phosphorelay circuitry appears to be propagated in response to cytokinin, as in the case of plants, as far as the immediate early responses were concerned. This cultured cell system might therefore provide us with an alternative means to further characterize the mechanisms underlying cytokinin (and also auxin) responses at the molecular level.

• 社団法人 日本農芸化学会の論文
• 2004-09-23

著者

• Kato Takahiko

  Kazusa Dna Research Institute

• KATO Tomohiko

  Kazusa DNA Research Institute

• Yamada Hideyuki

  SEIREN CO. LTD.

• YAMASHINO Takafumi

  Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University

• MIZUNO Takeshi

  Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University

• NAKAMICHI Norihito

  Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University

• Kiba Takatoshi

  Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University

• KOIZUMI Nobuya

  Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University

• YAMADA Hisami

  Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University

• KURIYAMA Tomoko

  RIKEN Plant Science Center

• 柿本 辰男

  Department Of Biology Graduate School Of Science Osaka University

• Nakamichi Norihito

  Riken Yokohama Jpn

• Nakamichi Norihito

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• Kinoue Tomoyo

  Kagoshima Univ. Kagoshima Jpn

• Mizuno Takeshi

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• Koizumi N

  Kyoto Univ. Kyoto Jpn

• Kato T

  Forestry Res. Inst. Oji Paper Co. Ltd.

• Kato Tomohiko

  Mitsui Plant Biotech. Res. Inst.

• Mizuno T

  Lab. Of Molecular Microbiology School Of Agriculture Nagoya Univ.

• Kato Takahiko

  Lab. Of Molecular Microbiology School Of Agriculture Nagoya Univ.

• Yamada Hidenori

  Department Of Bioengineering Science Faculty Of Engineering Okayama University

• Yamada H

  Institute For Bee Products And Health Science Yamada Apiculture Center Inc.

• Yamada Hisami

  Laboratory Of Microbiology School Of Agriculture Nagoya University

• Kiba T

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• Kiba Takatoshi

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• Yamada Hisami

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• Yamashino Takafumi

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• Koizumi N

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• Kondo Takao

  Graduate School Of Biological Sciences Nagoya University

• Mizuno Takeshi

  Laboratory Of Microbiology School Of Agriculture Nagoya University

• Yamada Hideo

  Institute For Bee Products And Health Science Yamada Apiculture Center Inc.

• YAMASHINO Takafumi

  Laboratory of Molecular and Functional Cenomics, School of Agriculture, Nagoya University

• NAKAMICHI Norihito

  Laboratory of Molecular and Functional Cenomics, School of Agriculture, Nagoya University

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