Occurrence of GalNAcβ1-4GlcNAc Unit in N-Glycan of Royal Jelly Glycoprotein(Biochemistry & Molecular Biology)

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概要

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• Elsewhere, we characterized the structure of twelve N-glycans purified from royal jelly glycoproteins (Kimura, Y. et al., Biosci. Biotechnol. Biochem., 64, 2109-2120 (2000)). Structural analysis showed that the typical high-mannose type structure (Man9-4GlcNAc2) accounts for about 72% of total N-glycans, a biantennary-type structure (GlcNAc2Man3GlcNAc2) about 8%, and a hybrid-type structure (GlcNAclMan4GlcNAc2) about 3%. During structural analysis of minor N-glycans of royal jelly glycoproteins, we found that one had an N-acetyl-galactosaminyl residue at the non reducing end ; most of such residues have been found in N-glycans of mammalian glycoproteins. By exoglycosidase digestion, methylation analysis, ion-spray (IS)-MS analysis, and ^1H NMR spectroscopy, we identified the structure of the N-glycan containing GalNAc as ; GlcNAcβ1-2Manα1-6(GaI-NAcβ1-4GlcNAcβ1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc. This result suggested that a β1-4 GalNAc transferase is present in hypopharyngeal and mandibular glands of honeybees.

• 社団法人日本農芸化学会の論文
• 2002-09-23

著者

• 木村 吉伸

  岡山大学農学部

• 木村 吉伸

  岡山大学 農

• Sugimoto Hiroyuki

  Yamada Apiculture Center, Inc.

• YAMADA Hideo

  Yamada Apiculture Center, Inc.

• KIMURA Yoshinobu

  Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• Yamada Hideyuki

  SEIREN CO. LTD.

• 木村 吉伸

  Dep. Of Biofunctional Chemistry Graduate School Of Natural Sci. And Technol. Okayama Univ.

• Kuroyanagi M

  Fac. Of Pharmaceutical Sciences At Kagawa Campus Tokushima Bunri Univ.

• 木村 吉伸

  岡山大学

• Kimura Mariko

  Department Of Breast Oncology Aichi Cancer Center Hospital

• Kimura Mariko

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• HAMA Yoichiro

  Department of Applied Biological Sciences, Faculty of Agriculture, Saga University

• OKIHARA Kiyoshi

  Yamada Apiculture Center Inc.

• Kimura Yoshinobu

  Department Of Agricultural Science Faculty Of Agriculture Okayama University

• Sugimoto Hiroyuki

  Yamada Apiculture Center

• Yamada Hidenori

  Department Of Bioengineering Science Faculty Of Engineering Okayama University

• TSUMURA Kazunori

  Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• Kuroyanagi Masanori

  Fac. Of Pharmaceutical Sciences At Kagawa Campus Tokushima Bunri Univ.

• Yamada Hisami

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• OKIHARA Kiyoshi

  Institute for Bee Products and Health Science, Yamada Apiculture Center, Inc.

• Okihara K

  Institute For Bee Products And Health Science Yamada Apiculture Center Inc.

• Hama Yoichiro

  Department Of Applied Biochemistry And Food Science Saga University

• Kimura Mariko

  Fac. Of Food Culture Dep. Of Nutrition Kurashiki Sakuyo Univ.

• Tsumura K

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Okihara Kiyoshi

  Institute For Bee Products And Health Science Yamada Apiculture Center Inc.

• Yamada Hideo

  Yamada Apiculture Center Inc.

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