Occurrence of Free N-Glycans in Pea (Pisum sativum. L) Seedlings
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概要
- 論文の詳細を見る
Free N-glycans have been found in pea seedlings. These free N-glycans were coupled with 2-aminopyridine and purified by gel filtration, Con A-Sepharose affinity chromatography, and size fractionation HPLC. These structures of pyridylaminated free N-glycans were analyzed by exomannosidase digestions and ionspray tandem mass spectrometry. The structural analyses showed that the several oligomannose-type sugar chains having one GlcNAc residue at the reducing-end side occur in the seedlings, suggesting the endo-β-N-acetylglucosaminidase PS [Y. Kimura et al., Biosci. Biotech. Biochem., 60, 228-232 (1996)] should be involved in the release of oligomannose-type N-glycans from the storage glycoproteins [Y. Kimura et al., Biosci. Biotech. Biochem., 60, 1841-1850(1996)] during the germination of pea seeds.
- 社団法人日本農芸化学会の論文
- 1997-05-23
著者
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KIMURA Yoshinobu
Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University
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Kimura Yoshinobu
Department Of Agricultural Science Faculty Of Agriculture Okayama University
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Shiraishi Tomonori
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
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TAKAGI Shigeaki
Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University
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Takagi Shigeaki
Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Takagi Shigeaki
Department Of Bioresources Chemistry Okayama University
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Kimura Yoshinobu
Department Of Bioresources Chemistry Okayama University
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Takagi Shigeaki
Department of Agricultural Chemistry, Faculty of Agriculture Okayama University
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