Glycinin A_4A_5 Subunit Digesting Protease in Soybean Seeds
スポンサーリンク
概要
- 論文の詳細を見る
Endopeptidase was partially purified from the globulin fraction of 4-hr-imbibed soybean seeds. The protease fraction obtained had proteolytic activity on the glycinin A_4A_5 subunit at both pH 4 and 8. A suitable peptidic substrate for the endopeptidase was isolated from the tryptic digest of the carboxymethylated A_4A_5 subunit. Using the tryptic peptide of glycinin A_5 subunit, a simple assay system for the soybean endopeptidase activity has been established. The activity was significantly inhibited by phenylmethylsulfonyl fluoride, indicating the endopeptidase is a serine protease.
- 社団法人日本農芸化学会の論文
- 1992-06-23
著者
-
KIMURA Yoshinobu
Division of Bioscience, Department of Biofunctional Chemistry, Graduate School of Natural Science an
-
TAKAGI Shigeaki
Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University
-
Takagi S
Okayama Univ. Okayama Jpn
-
Takagi Shigeaki
Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University
-
Akhtaruzzaman Mohammad
Department of Agricultural Science, Faculty of Agriculture, Okayama University
-
Akhtaruzzaman Mohammad
Department Of Agricultural Science Faculty Of Agriculture Okayama University
-
KIMURA Yoshinobu
Division of Anesthesia, Asahikawa Redcross Hospital
-
Takagi Shigeaki
Department of Agricultural Chemistry, Faculty of Agriculture Okayama University
関連論文
- Glycoform Analysis of N-Glycans Linked to Glycoproteins Expressed in Rice Culture Cells : Predominant Occurrence of Complex Type N-Glycans
- Glycoform Analysis of Japanese Cedar Pollen Allergen, Cry j 1
- Antiviral Activity of a Hot Water Extract of Black Soybean against a Human Respiratory Illness Virus(Food & Nutrition Science)
- Structural Analysis of N-Glycans of Storage Glycoproteins in Soybean (Glycine max. L) Seed
- Structural Elucidation of N-Linked Sugar Chains of Storage Glycoproteins in Mature Pea (Pisum sativum) Seeds by Ion-spray Tandem Mass Spectrometry (IS-MS/MS)
- Occurrence of Free N-Glycans in Pea (Pisum sativum. L) Seedlings
- Mechanism of Photoregulated Carotenogenesis in Rhodotorula minuta VIII. Effect of Mevinolin on Photoinduced Carotenogenesis
- Purification and Characterization of a CM-Glycinin Digesting Protease from Soybean Seeds
- Glycinin A_4A_5 Subunit Digesting Protease in Soybean Seeds
- Structures of N-Linked Oligosaccharides of Microsomal Glycoproteins from Developing Castor Bean Endosperms
- Primary Structures of N-Linked Oligosaccharides of Momordin-a, a Ribosome-inactivating Protein from Momordica charantia Seeds(Biological Chemistry)
- Structures of Sugar Chains of Water-soluble Glycoproteins in Developing Castor Bean Cotyledons(Biological Chemistry)
- Enzymatic Properties of a Ginkgo biloba Endo-β-N-acetylglucosaminidase and N-Glycan Structures of Storage Glycoproteins in the Seeds
- Structural Analyses of Sugar Chains from Ricin A-Chain Variant(Biological Chemistry)
- Purification and Substrate Specificity of an Endo-β-N-acetylglucosaminidase from Pea (Pisum sativum) Seeds
- Risk factors for gastric distension in patients with acute appendicitis : a retrospective cohort study
- Further Studies on the Acquisition of Novel Optical Activity on Interaction of Lutein and Other Carotenoids with Proteins
- Visible Circular Dichroism of Lutein Aggregate in a Cationic Surfactant Solution
- Visible Circular Dichroism of Lutein Acquired on Dispersion in an Aqueous Solution in the Presence of a Limited Amount of Sodium Dodecyl Sulfate and a Dramatic Change of the CD Spectrum with Concentration of the Surfactant
- Stopped-Flow Circular Dichroism Study on Conformational Change of Alpha-Chymotrypsin by Sodium Dodecyl Sulfate
- Lutein Dispersed in Acetone Aqueous Solution; Spectroscopic Analysis and Electron Microscope Observation
- Helical Configuration of Lutein Aggregate Dispersed in Liposomes of Phosphatidyl Choline and Digalactocyldiglyceride
- Determination of Green Leaf Carotenoids by HPLC
- Aggregation, Configuration and Particle Size of Lutein Dispersed by Sodium Dodecyl Sulfate in Various Salt Concentrations
- Effect of Addition of Lipids on the Novel Optical Activity of the Complex between Lutein and Ovalbumin