Glycoform Analysis of N-Glycans Linked to Glycoproteins Expressed in Rice Culture Cells : Predominant Occurrence of Complex Type N-Glycans
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概要
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Although it has been found that plant endo-β-N-acetylglucosaminidase shows strong activity towards denatured glycoproteins and glycopeptides with high-mannose type N-glycans and free high-mannose type N-glycans bearing the chitobiosyl unit, the endogenous substrates for plant endoglycosidase have not yet been identified. Recently we purified and characterized an endo-β-N-acetylglucosaminidase from rice culture cells and identified the gene encoded (Maeda, M., and Kimura, Y., Trends Glycosci. Glycotech., 17, 205–214 (2005)). Furthermore, we found structural features of free N-glycans in the cells, indicating that high-mannose type species (Man9-5GlcNAc1) occur at concentration of several micromolar (μM). Hence, in this study we analyzed glycoform of N-glycans linked to glycoproteins expressed in rice culture cells to see whether endogenous glycoproteinous substrate occurs in reasonable amounts. Structural analysis revealed that more than 95% of total N-glycans linked to glycoproteins in the rice cells had the plant complex type structure, including Lewis a epitope-harboring type, although high-mannose type structures account for less than 5% of total N-glycans.
- 社団法人 日本農芸化学会の論文
- 2006-06-23
著者
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木村 吉伸
岡山大学農学部
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木村 吉伸
岡山大学 農
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木村 吉伸
Dep. Of Biofunctional Chemistry Graduate School Of Natural Sci. And Technol. Okayama Univ.
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MAEDA Megumi
Division of Bioscience, Department of Biofunctional Chemistry, Graduate School of Natural Science an
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KIMURA Yoshinobu
Division of Bioscience, Department of Biofunctional Chemistry, Graduate School of Natural Science an
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木村 吉伸
岡山大学
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Maeda Megumi
Division Of Bioscience Department Of Biofunctional Chemistry Graduate School Of Natural Science And
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Kimura Yoshinobu
Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ
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MAEDA Megumi
Division of Biomolecular Science, Graduate School of Natural Science and Technology, Okayama University
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KIMURA Yoshinobu
Division of Anesthesia, Asahikawa Redcross Hospital
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