Structural Features of N-Glycans Linked to Glycoproteins from Oil Palm Pollen, an Allergenic Pollen(Biochemistry & Molecular Biology)

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概要

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• The pollen of oil palm (Elaeis guineensis Jacq.) is a strong allergen and causes severe pollinosis in Malaysia and Singapore. In the previous study (Biosci. Biotechnol Biochem., 64, 820-827 (2002)), from the oil palm pollens, we purified an antigenic glycoprotein (Ela g Bd 31 K), which is recognized by IgE from palm pollinosis patients. In this report, we describe the structural analysis of sugar chains linked to palm pollen glycoproteins to confirm the ubiquitous occurrence of antigenic N-glycans in the allergenic pollen. N-Glycans liberated from the pollen glycoprotein mixture by hydrazinolysis were labeled with 2-aminopyridine followed by purification with a combination of size-fractionation HPLC and reversed-phase HPLC. The structures of the PA-sugar chains were analyzed by a combination of two-dimensional sugar chain mapping, electrospray ionization mass spectrometry (ESI-MS), and tandem MS analysis, as well as exoglycosidase digestions. The antigenic N-glycan bearing α1-3 fucose and/or β1-2 xylose residues accounts for 36.9% of total N-glycans : GlcNAc_2Man_3Xyl_1Fuc_1GlcNAc_2 (24.6%), GlcNAc_2Man_3Xyl_1GlcNAc_2 (4.4%), Man_3Xyl_1Fuc_1-GlcNAc2 (1.1%), GlcNAc_1Man_3Xyl_1Fuc_1GlcNAc_2 (5.6%), and GlcNAc_1Man_3Xyl_1GlcNAc_2 (1.2%). The remaining 63.1% of the total N-glycans belong to the high-mannose type structure : Man9GlcNAc2 (5.8%), Man8GlcNAc2 (32.1%), Man7GlcNAc2 (19.9%), Man6GlcNAc2 (5.3%).

• 社団法人日本農芸化学会の論文
• 2003-10-23

著者

• 木村 吉伸

  岡山大学農学部

• 木村 吉伸

  岡山大学 農

• KIMURA Yoshinobu

  Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• 木村 吉伸

  Dep. Of Biofunctional Chemistry Graduate School Of Natural Sci. And Technol. Okayama Univ.

• MAEDA Megumi

  Division of Bioscience, Department of Biofunctional Chemistry, Graduate School of Natural Science an

• Kuroyanagi M

  Fac. Of Pharmaceutical Sciences At Kagawa Campus Tokushima Bunri Univ.

• 木村 吉伸

  岡山大学

• Kimura Mariko

  Department Of Breast Oncology Aichi Cancer Center Hospital

• Kimura Mariko

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Kimura M

  Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University

• Maeda Megumi

  Division Of Bioscience Department Of Biofunctional Chemistry Graduate School Of Natural Science And

• YOSHIIE Takeo

  Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• KIT Woo

  Division of Biomolecular Science, The Graduate School of Natural Science and Technology, Okayama Uni

• TAN Siang

  Genome Centre, Institute Bioscience, Universiti Putra Malaysia

• Kimura Yoshinobu

  Department Of Agricultural Science Faculty Of Agriculture Okayama University

• Kit Woo

  Division Of Biomolecular Science The Graduate School Of Natural Science And Technology Okayama Unive

• Tan Siang

  Genome Centre Institute Bioscience Universiti Putra Malaysia

• Yoshiie Takeo

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Kuroyanagi Masanori

  Fac. Of Pharmaceutical Sciences At Kagawa Campus Tokushima Bunri Univ.

• Yoshiie Takeo

  Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ

• Kimura Mariko

  Fac. Of Food Culture Dep. Of Nutrition Kurashiki Sakuyo Univ.

• Maeda M

  Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ

• MAEDA Megumi

  Division of Biomolecular Science, Graduate School of Natural Science and Technology, Okayama University

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