Isolated Ricin B-Chain-mediated Apoptosis in U937 Cells
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概要
- 論文の詳細を見る
We have previously reported that ricin, a toxic lectin that inhibits protein synthesis induced apoptotic cell death. In this study, we have found that isolated ricin CM-B-chain, which has no effect on cellular protein synthesis, induced DNA fragmentation in U937 cells in a dose-and time-dependent manner, albeit it required a longer incubation time and higher concentration than those of holotoxin ricin. Z-Asp-CH_2-DCB, a caspase family inhibitor and serine protease inhibitor, 3,4-dichloroisocoumarine (DCI) effectively inhibited the CM-B-chain-mediated DNA fragmentation as well as in ricin. Thus, like ricin, multiple proteases with different substrate specificity may also be involved in the CM-B-chain-mediated apoptotic pathway. Furthermore, BFA inhibited both ricin-and CM-B-chain-mediated DNA fragmentation, suggesting an intracellular vesicle transport system through the Golgi complex may be involved in the apoptotic induction by these proteins as a common feature. On the other hand, cycloheximide (CHA) strongly increased the CM-B-chain-mediated DNA fragmentation, but inhibited ricin-mediated DNA fragmentation. The opposite effects of CHA may reflect the difference in the apoptotic mechanism between ricin and CM-B-chain. In conclusion, our results suggest that ricin-B-chain can induce apoptosis through its lectin activity, but the underlying mechanism may be distinct from that of ricin in which the A-chain contributes profoundly to the apoptotic induction.
- 社団法人日本農芸化学会の論文
- 2000-07-23
著者
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Oda Tatsuya
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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Oda Tatsuya
Div. Of Biochemistry Fac. Of Fisheries Nagasaki Univ.
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Oda T
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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KIMURA Yoshinobu
Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University
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Tatsuya Oda
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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Oda T
Nagasaki Univ. Nagasaki Jpn
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Oda T
New Industry Creation Hatchery Center Tohoku University
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MURAMATSU Tsuyoshi
Division of Biochemistry, Faculty of Fisheries, Nagasaki University
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Okamoto Tarou
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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KOMATSU Nobukazu
Division of Biochemistry, Faculty of Fisheries, Nagasaki University
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HASEGAWA Naoko
Division of Biochemistry, Faculty of Fisheries, Nagasaki University
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Kimura Yoshinobu
Department Of Agricultural Science Faculty Of Agriculture Okayama University
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Muramatsu Tsuyoshi
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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Muramatsu Tsuyoshi
Division Of Biochemistry Faculty Of Fisheries Nagasaki University:(present Address)department Of Foo
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Muramatsu T
Division Of Biochemistry Faculty Of Fisheries Nagasaki University:(present Address)department Of Foo
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Hasegawa Naoko
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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Komatsu Nobukazu
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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