Concanavalin A-Induced Discharge of Glycocalyx of Raphidophycean Flagellates, Chattonella marina and Heterosigma akashiwo

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概要

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• Chattonella marina and Heterosigma akashiwo, known as red tide phytoplankton, are naturally wall-less and have quite fragile cell structures. In this study, we found that an equilibrium dialysis technique allowed the study of lectin binding to these flagellates. The results suggested that concanavalin A (Con A) binds to these flagellate cells through the specific carbohydrate moieties on the cell surface. Interestingly, the binding of an excess of Con A on the cell surface caused morphological changes concomitant with discharge of glycocalyx, a polysaccharide-containing common structure on the external cell surface of these flagellates. Fluorescent microscopic observation using FITC-labeled Con A (F-Con A) confirmed that F-Con A molecules are localized on the discharged glycocalyx.

• 社団法人日本農芸化学会の論文
• 2000-08-23

著者

• Oda Tatsuya

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Oda Tatsuya

  Div. Of Biochemistry Fac. Of Fisheries Nagasaki Univ.

• Oda T

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Tatsuya Oda

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Oda T

  Nagasaki Univ. Nagasaki Jpn

• Kim D

  Jeju Center Korea Basic Science Institute (kbsi)

• ISHIMATSU ATSUSHI

  Institute for East China Sea Research, Nagasaki University

• Ishimatsu A

  Nagasaki Univ. Nagasaki

• Ishimatsu Atsushi

  Inst. For East China Sea Res. Nagasaki Univ.

• Ishimatsu Atsushi

  Marine Research Institute Nagasaki University

• Oda T

  New Industry Creation Hatchery Center Tohoku University

• Kim Daekyung

  Division of Biochemistry, Faculty of Fisheries, Nagasaki University

• MURAMATSU Tsuyoshi

  Division of Biochemistry, Faculty of Fisheries, Nagasaki University

• OKAMOTO Tarou

  Division of Biochemistry, Faculty of Fisheries, Nagasaki University

• MATSUOKA Kazumi

  Laboratory of Coastal Environmental Sciences, Faculty of Fisheries, Nagasaki University

• Okamoto Tarou

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Kim Daekyung

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Ishimatsu Atsushi

  Noma Fisheries Station Nagasaki University

• Muramatsu Tsuyoshi

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Muramatsu Tsuyoshi

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University:(present Address)department Of Foo

• Muramatsu T

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University:(present Address)department Of Foo

• Okamoto T

  Department Of Agricultural Chemistry Faculty Of Agriculture Kinki University

• Matsuoka K

  Institute Of East China Sea Research Nagasaki University

• Matsuoka Kazumi

  Laboratory Of Coastal Environmental Sciences Faculty Of Fisheries Nagasaki University

• Matsuoka Kazumi

  Department Of Marine Biology Faculty Of Fisheries Nagasaki University

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