Cell Lysis Induced by Ricin D and Ricin E in Various Cell Lines
スポンサーリンク
概要
- 論文の詳細を見る
Ricin D, one of two isolectins from small castor beans showed stronger cytotoxicity than another one, ricin E, based on the inhibition of colony formation and the inhibition of protein synthesis. Both ricin D and ricin E induced cell lysis to different extents in each cell line tested, albeit ricin E was slightly less effective than ricin D. DNA fragmentation, a characteristic feature of apoptosis, was also induced by ricin D and ricin E in Vero cells. Scatchard plot analysis showed that ricin D binds to celles with higher affinity than ricin E, while the number of binding sites per cell was not much different, suggesting that the differences in the cytotoxicity between ricin D and ricin E is mainly due to their differential binding affinity to cells. In Vero cells, the cytolytic activities of ricin D and ricin E were inhibited by brefeldin A (BFA), which is known to affect the Golgi apparatus, but no significant effect of BFA was observed in a BFA-resistant cell line, MDCK cells. These results suggest that the Golgi apparatus may be involved in ricin-induced cell lysis.
- 社団法人日本農芸化学会の論文
- 1997-02-23
著者
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Oda Tatsuya
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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Oda T
Nagasaki Univ. Nagasaki Jpn
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MURAMATSU Tsuyoshi
Division of Biochemistry, Faculty of Fisheries, Nagasaki University
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KOMATSU Nobukazu
Division of Biochemistry, Faculty of Fisheries, Nagasaki University
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Muramatsu Tsuyoshi
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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Muramatsu Tsuyoshi
Division Of Biochemistry Faculty Of Fisheries Nagasaki University:(present Address)department Of Foo
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Muramatsu T
Division Of Biochemistry Faculty Of Fisheries Nagasaki University:(present Address)department Of Foo
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Komatsu Nobukazu
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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