Involvement of N-Acetylcysteine-sensitive Pathways in Ricin-induced Apoptotic Cell Death in U937 Cells

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概要

• 論文の詳細を見る

• We have found that the antioxidant N-acetylcysteine (NAC) strongly inhibited ricin-induced apoptotic cell death in U937 cells (human myeloid leukemia), as judged by cytotoxicity, nuclear morphological change, and DNA fragmentation. Consistent with these observations, a significant depletion of cellular glutathione was observed in ricin-treated cells, and NAC prevented the decrease in cellular glutathione. On the other hand, among the caspase inhibitors tested, Z-Asp-CH_2-DCB, which inhibited ricin cytotoxicity, also suppressed ricin-mediated glutathione depletion, while NAC did not affect the generation of caspase-3 like activity in ricin-treated cells. These results suggest that glutathione loss takes place downstream from caspase activation during the ricin-induced apoptotic process. Treatment with a specific inhibitor of glutathione biosynthesis, buthionine sulfoximine (BSO) failed to induce apoptosis, and had no effect on the overall extent of ricin-induced apoptosis, even though the glutathione level was decreased to less than 5% of the control level. However, NAC still protected against ricin-induced apoptosis in the BSO-treated cells. We conclude that glutathione loss is one of several apoptotic changes caused by ricin, but is not a sufficient factor for the progress of apoptosis. NAC may prevent ricininduced apoptosis through maintaining an intracellular reducing condition by acting as a thiol supplier.

• 社団法人日本農芸化学会の論文
• 1999-02-23

著者

• Oda Tatsuya

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Oda Tatsuya

  Div. Of Biochemistry Fac. Of Fisheries Nagasaki Univ.

• Oda T

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Tatsuya Oda

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Oda T

  Nagasaki Univ. Nagasaki Jpn

• Oda T

  New Industry Creation Hatchery Center Tohoku University

• MURAMATSU Tsuyoshi

  Division of Biochemistry, Faculty of Fisheries, Nagasaki University

• Okamoto Tarou

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• KOMATSU Nobukazu

  Division of Biochemistry, Faculty of Fisheries, Nagasaki University

• IWAOKA June

  Division of Biochemistry, Faculty of Fisheries, Nagasaki University

• Muramatsu Tsuyoshi

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Muramatsu Tsuyoshi

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University:(present Address)department Of Foo

• Muramatsu T

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University:(present Address)department Of Foo

• Iwaoka June

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Komatsu Nobukazu

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

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