Cloning and Sequence Analyais of a Gene (bly PG) Encoding Poly(α-L-guluronate)lyase from Corynebacterium sp.Strain ALY-1

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概要

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• The aly PG gene, coding for a poly α-_L-guluronate lyase (PG lyase) of Corynebacterium strain ALY-1, was cloned and sequenced. The gene consists of 768 bp encoding a signal peptide of 32 amino acids and a mature protein of 224 amino acids. Two disulfide bond cross-linkages were found to be formed between Cys-4 and Cys-51 and between Cys-200 and Cys-206 in the native PG lyase molecule. The deduced amino acid sequence of the Corynebacterium sp. aly PG gene exhibited 29% homology toward that of the Klebsiella pneumoniae, subsp. aerogenes aly A gene, with two conserved regions (the amino acid sequences from Y-102 to M-110 and from Y-221 to Q-229).

• 社団法人日本生物工学会の論文
• 2000-02-25

著者

• Oda Tatsuya

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• MURAMATSU Tsuyoshi

  Division of Biochemistry, Faculty of Fisheries, Nagasaki University

• Iwasaki K

  Food Research Institute Kagawa Prefectural Government

• Iwasaki Ken-ichi

  Food Research Institute Kagawa Prefectural Government

• Iwasaki Ken-ichi

  Food Research Institute Kagawa Perfectural Government

• MATSUBARA YASUHITO

  Kagawa Prefectural Fermentation and Food Experimental Station

• KAWADA RYOKO

  Division of Biochemistry, Faculty of Fisheries, Nagasaki University

• KIMURA YOSHIO

  Department of Life Science, Faculty of Agriculture, Kagawa University

• Muramatsu Tsuyoshi

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Kawada Ryoko

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Matsubara Yasuhito

  Kagawa Prefectural Fermentation & Food Experimental Station

• Kimura Yoshio

  Department Of Applied Biological Science Faculty Of Agriculture Kagawa University

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