Purification of Alginate Oligosaccharides with Root Growth-promoting Activity toward Lettuce
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概要
- 論文の詳細を見る
Sodium alginate was degraded by alginate lyase from Corynebacterium sp., and the product was purified by an ultrafiltration (UF) membrane module. The UF treatment was carried out at a transmembrane pressure of 0.15 MPa and a flow velocity of 0.6m/s in the crossflow mode, and non-degraded alginate was almost completely removed. The alginate oligosaccharide obtained was a mixture of di-to octasaccharides and had promoting activity toward lettuce root elongation (about 2-fold compared with the control) in the concentration range of 200-3000μg/ml. The effect of the degree of polymerization on this activity was examined by using each oligosaccharide fractionated by gel chromatography. The tri-, tetra-, penta-and hexasaccharides were each found to have root growth-promoting activity in a lettuce bioassay.
- 社団法人日本農芸化学会の論文
- 2000-05-23
著者
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Iwasaki Ken-ichi
Food Research Institute Kagawa Perfectural Government
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Matsubara Yasuhito
Kagawa Prefectural Fermentation & Food Experimental Station
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Matsubara Yasuhito
Kagawa Prefectural Fermentation And Food Experimental Station:(present Address)food Res. Inst. Kagaw
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Iwasaki Kenichi
Food Research Institute Kagawa Prefectural Government
関連論文
- Purification of Pectate Oligosaccharides Showing Root-Growth-Promoting Activity in Lettuce Using Ultrafiltration and Nanofiltration Membranes
- Cloning and Sequence Analyais of a Gene (bly PG) Encoding Poly(α-L-guluronate)lyase from Corynebacterium sp.Strain ALY-1
- Continuous Hydrolysis of Pectate by Immobilized Endo-polygalacturonase in a Continuously Stirred Tank Reactor
- Recovery of Oligosaccharides from Steamed Soybedn Waste Water in Tofu Processing by Reverse Osmosis and Nanofiltration Membranes
- Effective Moisture Diffusivity of Fresh Japanese Noodle (udon) as a Function of Temperature
- Purification of Alginate Oligosaccharides with Root Growth-promoting Activity toward Lettuce
- Action of Poly (α-L-guluronate) lyase from Corynebacterium sp. ALY-1 strain on Saturated Oligoguluronates
- Continuous Degradation of Sodium Alginate in Bioreactor Using Immobilized Alginate Lyase.