Effects of sulfated fucan, ascophyllan, from the brown Alga Ascophyllum nodosum on various cell lines: a comparative study on ascophyllan and fucoidan.

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概要

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• The effects of fucose-containing sulfated polysaccharides, ascophyllan and fucoidan, isolated from the brown alga Ascophyllum nodosum, on the growth of various cell lines (MDCK, Vero, PtK(1), CHO, HeLa, and XC) were investigated. In a colony formation assay, ascophyllan and fucoidan showed potent cytotoxic effects on Vero and XC cells, while other cell lines were relatively resistant to these polysaccharides. Almost no significant effects of these polysaccharides were observed in the cell lines tested using the Alamar blue cytotoxicity assay over 48 h with varying initial cell densities (2500-20,000 cells/well) in growth medium. Interestingly, a significant growth promoting effect of ascophyllan on MDCK cells was observed, whereas treatment with fucoidan showed growth suppressive effects on this cell line under the same experimental conditions. These results suggest that ascophyllan is distinguishable from fucoidan in terms of their bioactivities. This is the first report of the growth promoting effects of a sulfated fucan on a mammalian cell line under normal growth conditions.

著者

• Oda Tatsuya

  Div. Of Biochemistry Fac. Of Fisheries Nagasaki Univ.

• Oda T

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Jiang Zedong

  Graduate School of Science and Technology, Nagasaki University

• Okimura Takasi

  Research and Development Division, Hayashikane Sangyo Co., Ltd.

• Yokose Takeshi

  Graduate School of Science and Technology, Nagasaki University

• Yamasaki Yasuhiro

  Division of Biochemistry, Faculty of Fisheries, Nagasaki University

• Yamaguchi Kenichi

  Graduate School of Science and Technology, Nagasaki University

• Oda Tatsuya

  Graduate School of Science and Technology, Nagasaki University

• YAMAGUCHI Kenichi

  Division of Biochemistry, Faculty of Fisheries, Nagasaki University

• Yamaguchi Kenichi

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Tatsuya Oda

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Oda T

  Nagasaki Univ. Nagasaki Jpn

• Oda T

  New Industry Creation Hatchery Center Tohoku University

• JIANG Zedong

  Division of Biochemistry, Faculty of Fisheries, Nagasaki University

• Jiang Zedong

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Okamoto Tarou

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Okimura Takasi

  Research And Development Division Hayashikane Sangyo Co. Ltd.

• Yokose Takeshi

  Nagasaki Prefectural Institute for Environment Research and Public Health

• Yamamoto Yoshiko

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Yamasaki Yasuhiro

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Oda T

  Faculty Of Fisheries Nagasaki University

• Yokose Takeshi

  Graduate School Of Science And Technology Nagasaki University:nagasaki Prefectural Institute For Env

• Yamaguchi Kenichi

  New Industry Creation Hatchery Center Tohoku University

• Oda Tatsuya

  Graduate School Of Science And Technology Nagasaki University

• Yamaguchi Kenichi

  Graduate School Of Science And Technology Nagasaki University

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