Structures of N-Linked Oligosaccharides of Glycoproteins from Tobacco BY2 Suspension Cultured Cells

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概要

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• The structures of N-linked sugar chains of glycoproteins expressed in tobacco BY2 cultured cells are reported. Five pyridylaminated (PA-) N-linked sugar chains were derived and purified from hydrazinolysates of the glycoproteins by reversed-phase HPLC and size-fractionation HPLC. The structures of the PA-sugar chain mapping, ion-spray MS/MS analysis, and exoglycosidase digestions. The five structures fell into two categories ; the major class (92.5% as molar ratio) was a xylose containing-type (Man_3Fuc_1Xyl_1GlcNAc_2 (41.0%), GlcNAc_2Man_3Fuc_1Xyl_1GlcNAc_2 (26.5%), GlcNAc_1Man_3Fuc_1Xyl_1GlcNAc_2 (21.7%), Man_3Xyl_1GlcNAc_2 (3.3%)), and the minor class was a high-man-nose type (Man_5GlcNAc_2 (7.5%)). This is the first report to show that α (1→3) fucosylation of N-glycans does occur but β (1→4) galactosylation of the sugar chains does not in the tobacco cultured cells.

• 社団法人日本農芸化学会の論文
• 1999-01-23

著者

• Fujiyama Kazuhito

  International Center for Biotechnology, Osaka University

• Fujiyama Kazuhito

  The International Center for Biotechnology, Osaka University

• Kimura Yoshinobu

  The International Center For Biotechnology Osaka University

• KIMURA Yoshinobu

  Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• SEKI Tatsuji

  The International Center for Biotechnology, Osaka University

• PALACPAC NirianneQ.

  The International Center for Biotechnology, Osaka University

• Palacpac Nirianne

  The International Center for Biotechnology, Osaka University

• SEKI Tatsuji

  International Center for Biotechnology, Osaka University

• Kazuhito Fujiyama

  International Center For Biotechnology Osaka University

• Fujiyama Kazuhito

  大阪大学生物工学国際交流センター

• Fujiyama Kazuhito

  International Center For Biotechnology Osaka Univ.

• Fujiyama Kazuhito

  Department Of Fermentation Technology Osaka University

• Fujiyama Kazuhito

  International Center For Biotechnology Osaka University

• Seki Tatsuji

  International Center For Biotechnology Osaka University

• Seki Tatsuji

  The International Center For Biotechnology Osaka University

• Seki Tatsuji

  大阪大学生物工学国際交流センター

• Yoshida Toshiomi

  The International Center For Biotechnology Osaka University

• 吉田 豊和

  岐阜大・工・生命工

• Kimura Y

  Department Of Biological And Environmental Chemistry Faculty Of Agriculture Tottori University

• Kimura Yoshinobu

  Department Of Agricultural Science Faculty Of Agriculture Okayama University

• Palacpac Nirianneq.

  The International Center For Biotechnology Osaka University

• Fujiyama Kazuhito

  The International Center For Biotechnology Osaka Univ. 2-1 Yamada-oka Suita Osaka 565-0871 Jpn

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