Characterization of Almond α-Mannosidase and Its Application for Structure Analysis of Sugar Chain(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

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概要

• 論文の詳細を見る

• Almond α-mannosidase was purified by separation on columns of DEAE-Sephadex A50 and hydroxyapatite, and characterized. Its optimum pH was approximately 3.8. It was also shown to be stable from pH 6 to 8. Its activity was stable up to 60℃. The thermostability of almond α-mannosidase at 73℃ appeared to be superior to that of jack bean α-mannosidase. We examined the substrate specificity of the former toward high-mannose-type N-glycan Man_9GlcNAc_2, and showed that the deduced trimming pathway was more diverse than that of the latter. We could use almond α-mannosidase as well as jack bean α-mannosidase for analysis of sugar chain structures.

• 社団法人日本生物工学会の論文
• 2003-08-25

著者

• Fujiyama Kazuhito

  International Center for Biotechnology, Osaka University

• Fujiyama Kazuhito

  The International Center for Biotechnology, Osaka University

• MISAKI Ryo

  The International Center for Biotechnology, Osaka University

• SEKI Tatsuji

  The International Center for Biotechnology, Osaka University

• Yoshida Toshiomi

  The International Center for Biotechnology, Osaka University

• SEKI Tatsuji

  International Center for Biotechnology, Osaka University

• IDO YOSHIHIRO

  The International Center for Biotechnology, Osaka University

• Kazuhito Fujiyama

  International Center For Biotechnology Osaka University

• Fujiyama Kazuhito

  大阪大学生物工学国際交流センター

• Fujiyama Kazuhito

  International Center For Biotechnology Osaka Univ.

• Fujiyama Kazuhito

  Department Of Fermentation Technology Osaka University

• Fujiyama Kazuhito

  International Center For Biotechnology Osaka University

• Misaki Ryo

  International Center For Biotechnology Osaka University

• Misaki Ryo

  The International Center For Biotechnology Osaka University

• Seki Tatsuji

  International Center For Biotechnology Osaka University

• Seki Tatsuji

  The International Center For Biotechnology Osaka University

• Seki Tatsuji

  大阪大学生物工学国際交流センター

• Ido Yoshihiro

  The International Center For Biotechnology Osaka University

• Yoshida Toshiomi

  大阪大学生物工学国際交流センター

• Yoshida Toshiomi

  International Center Of Cooperative Research In Biotechnology Faculty Of Engineering Osaka Universit

• Yoshida Toshiomi

  The International Center For Biotechnology Osaka University

• Yoshida T

  International Center For Biotechnology Osaka University

• 吉田 豊和

  岐阜大・工・生命工

• Yoshida T

  Division Of Material And Biological Graduate School Japan Women's University

• YOKOYAMA HIROYUKI

  The International Center for Biotechnology, Osaka University

• MIYAUCHI KIYOTAKA

  The International Center for Biotechnology, Osaka University

• Yoshida T

  Kinki Univ. Nara Jpn

• Yoshida Toyokazu

  Dept. Food Nutri. Fac. Agr. Kinki Univ.

• Miyauchi Kiyotaka

  The International Center For Biotechnology Osaka University

• Yokoyama H

  The International Center For Biotechnology Osaka University

• Fujiyama Kazuhito

  The International Center For Biotechnology Osaka Univ. 2-1 Yamada-oka Suita Osaka 565-0871 Jpn

• Yoshida T

  Department Of Biomolecular Science Gifu University

• Yokoyama Hiroyuki

  The International Center For Biotechnology Osaka University

• 吉田 豊和

  岐阜大 工

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