Expanded Bed Adsorption for Purification of Alcohol Dehydrogenase Using a Dye-Iminodiacetic Acid Matrix (BIOCHEMICAL ENGINEERING)

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概要

• 論文の詳細を見る

• Expanded bed adsorption (EBA) was successfully applied for the purification of alcohol dehydrogenase (ADH) from unclarified bakers' yeast homogenate using a new dye-iminodiacetic acid (IDA) matrix carrying immobilized metal ions. The existence of cells and cell debris do not have a significant effect on the adsorption of ADH on the matrix. The presence of imidazole in adsorption buffer was found to be an important factor in reducing the level of the adsorbed contaminant proteins. The optimal imidazole concentration was 5 mM. The dynamic binding capacity of the dye-IDA matrix in EBA was found to be 569 U/ml matrix. A chromatography of one-step elution with imidazole was developed, in which ADH was successfully adsorbed in the presence of 5 mM imidazole; subsequently, the column was washed with 5 mM imidazole containing 1 M NaCl and eluted with 150 mM imidazole containing 1.5 M NaCl. The purification factor and ADH recovery were 8.8 and 93.5%, respectively.

• 社団法人日本生物工学会の論文
• 2004-04-25

著者

• TAKAGI Mutsumi

  International Center for Biotechnology, Osaka University

• YOSHIDA Toshiomi

  International Center for Biotechnology, Osaka University

• Yoshida Toshiomi

  大阪大学生物工学国際交流センター

• Yoshida Toshiomi

  International Center Of Cooperative Research In Biotechnology Faculty Of Engineering Osaka Universit

• Yoshida T

  International Center For Biotechnology Osaka University

• Yoshida Toshiomi

  International Center For Biotechnology Osaka University

• 吉田 豊和

  岐阜大・工・生命工

• Takagi Mutsumi

  International Center For Biotechnology Osaka University:(present Address)division Of Biotechnology A

• Takagi M

  International Center For Biotechnology Osaka University

• Takagi M

  Japan Advanced Inst. Of Sci. And Technol. Ishikawa Jpn

• Takagi M

  Osaka Univ. Osaka Jpn

• Hidayat Chusnul

  International Center For Biotechnology Osaka University

• Yoshida T

  Division Of Material And Biological Graduate School Japan Women's University

• Yoshida T

  Kinki Univ. Nara Jpn

• Yoshida Toyokazu

  Dept. Food Nutri. Fac. Agr. Kinki Univ.

• Takagi Mutsumi

  Internatioal Center For Biotechnology Osaka University

• Takagi Mutsumi

  School Of Materials Science Japan Advanced Institute Of Science And Technology

• Yoshida T

  Department Of Biomolecular Science Gifu University

• Yoshida Toshiomi

  Internatioal Center For Biotechnology Osaka University

• 吉田 豊和

  岐阜大 工

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