Control of Cell Growth Rate by Sugar Feeding Based on CO_2 Production Rate
スポンサーリンク
概要
- 論文の詳細を見る
Sugar feeding based on the CO_2 production rate was investigated for the control of the cell growth rate during cephalosporin C fermentation in which sugar concentration was the growth limiting factor. The rates of cell growth and sugar consumption were apparently influenced by the sugar feed rate, as the sugar concentration in the broth was kept at approximately 1g/l during the cultivation. The ratio of the CO_2 production rate to the sugar consumption rate was maintained almost constant after 40 h cultivation. Stepwise alteration in sugar feed rate after the culture time of 50 h caused a change in the CO_2 production rate within 15 min. These results indicated that the CO_2 production rate could be used as an effective parameter of sugar consumption and cell growth rates. Consequently, a control strategy was developed that involved the control of sugar feed rate so that a CO_2 production rate profile corresponded to a preset standard profile. For the half the usual amount of inoculum, the sugar feeding control system enabled the cell concentration to increase faster to that in the usual inoculum. The cell concentration deviation during exponential growth phase among several batches, decreased to half using this sugar feeding control system compared to the culture without such control. These results indicated that the sugar feeding control system developed in this report was found to be efficient for control of cell growth.
- 社団法人日本生物工学会の論文
- 1998-03-25
著者
-
TAKAGI Mutsumi
International Center for Biotechnology, Osaka University
-
Takagi Mutsumi
Pharmaceutical Research And Development Department Asahi Chemical Industry Company Ltd.:(present Add
-
ISHIMURA FUMIHIRO
Pharmaceutical Research and Development Department, Asahi Chemical Industry Company Ltd.
-
FUJIMATSU ISAO
Pharmaceutical Research and Development Department, Asahi Chemical Industry Company Ltd.
-
Fujimatsu I
Asahi Chemical Ind. Co. Ltd. Miyazaki Jpn
-
Takagi M
Asahi Chemical Industry Co. Ltd. Shizuoka Jpn
-
Maeda Takuya
Department Of Biotechnology Faculty Of Eigineering Osaka University
-
Ishimura Fumihiro
Pharmaceutical Research And Development Department Asahi Chemical Industry Company Ltd.
-
Fujimatsu Isao
Pharmaceutical Research And Development Department Asahi Chemical Industry Co. Ltd.
-
Takagi M
Japan Advanced Inst. Sci. And Technol. Ishikawa Jpn
関連論文
- Effect of heparin addition on expansion of cord blood hematopoietic progenitor cells in three-dimensional coculture with stromal cells in nonwoven fabrics
- Analysis of Hematopoietic Microenvironment Containing Spatial Development of Stromal Cells in Nonwoven Fabrics (ANIMAL BIOTECHNOLOGY)
- Control of Cell Growth Rate by Sugar Feeding Based on CO_2 Production Rate
- Production of S-(+)-Ibuprofen with High Optical Purity from a Nitrile Compound by Cells immobilized on Cellulose Porous Beads
- Production of S-(+)-Ibuprofen from a Nitrile Compound by Enzymatic Reaction Combined with Ultrafiltration
- Transduction of Static Pressure Signal to Expression of Human Granurocytes Macrophage Colony Stimulating Factor Mrna in Chinese Hamster Ovary Cells (ANIMAL BIOTECHNOLOGY)
- Effect of Static Pressure on Human Granulocyte-Macrophage Stimulating Factor (hGM-CSF) Production by Chinese Hamster Ovary Cells
- Effects of Shifts Up and Down in Osmotic Pressure on Production of Tissue Plasminogen Activator by Chinese Hamster Ovary Cells in Suspension
- Effect of chondroitin sulfate and hyaluronic acid on gene expression in a three-dimensional culture of chondrocytes
- Effect of Poly DL-Lactic-Co-Glycolic Acid Mesh on a Three-Dimensional Culture of Chondrocytes(CELL AND TISSUE ENGINEERING)
- Bacterial Alginate Lyase : Enzymology, Genetics and Application
- Hydrodynamically stable adhesion of endothelial cells onto a polypropylene hollow fiber membrane by modification with adhesive protein
- Enhanced adhesion of endothelial cells onto a polypropylene hellow-fiber membrane by plasma discharge treatment and high inoculum cell density
- Production of Bacterial Alginate-Specific Lyase by Recombinant Bacillus subtilis
- Crystallization and Preliminary X-Ray Diffraction Studies of Bacterial Alginate Lyase
- Metabolic Flux Change in Hybridoma Cells under High Osmotic Pressure
- 524 Improvement of Monoclonal Antibody Production with Gradual Increase of Osmotic Pressure
- Effect of Insoluble Medium Component on the Productivity of Tissue Plasminogen Activator by Cells on Microcarriers
- An Oxygen Supply Strategy for the Large-Scale Production of Tissue Plasminogen Activator by Microcarrier Cell Culture
- Effect of Sugar Residues in a Glycolipid Coated onto a Dish on Ammonia Consumption and Gluconeogenesis Activity of Primary Rat Hepatocytes
- Synthesis and Evaluation of Novel Peptide-Lipid
- Effect of galactose residue in glycolipid coated onto a dish on ammonia consumption activity of primary rat hepatocytes
- Expanded Bed Adsorption for Purification of Alcohol Dehydrogenase Using a Dye-Iminodiacetic Acid Matrix (BIOCHEMICAL ENGINEERING)
- Multivalent Binding Interaction of Alcohol Dehydrogenase on Dye-Metal Affinity Matrix(BIOCHEMICAL ENGINEERING)
- Development of New Dye-Metal Agarose-Coated Alumina Matrix and Elution Strategy for Purification of Alcohol Dehydrogenase
- 336 Development of polyethyleneimine-coated alumina for dye-affinity chromatography
- The Human-Specific Action of Intermedilysin, a Homolog of Streptolysin O, Is Dictated by Domain 4 of the Protein
- Synthesis and Biological Properties of Gemini Quaternary Ammonium Compounds, 5,5'-[2,2'-(α,ω-Polymethylnedicarbonyldioxy)diethyl]bis-(3-alkyl-4-methylthiazolium iodide) and 5,5'-[2,2'-(p-Phenylenedicarbonyldioxy)diethyl]bis(3-alkyl-4-methylthiazolium brom
- Antifungal Characteristics of N,N'-Hexamethylenebis (4-carbamoyl-1-decylpyridinium bromide)
- Synthesis of 4, 4'-(Tetramethylenedicarbonyldiamino)-bis(1-decylpyridinium bromide) and Its Antimicrobial and Deodorant Characteristics
- Synthesis and Antimicrobial Characteristics of N, N'-Hexamethylenebis(4-carbamoyl-1-decylpyridinium bromide
- Synthesis and Antimicrobial Characteristics of 5, 5'-[2, 2'-(Tetramethylenedicarbonyldioxy) diethyl] bis(3-alkyl-4-methylthiazolium iodide)s
- Synthesis and Antimicrobial Characteristics of Novel Biocides, 4,4'-(1,6-Hexamethylenedioxydicarbonyl)bis(1-alkylpyridinium iodide)s
- Synthesis and Bactericidal Characteristics of N-Alkyl-2-benzylaminopyridinium Iodides
- Repeated Addition of Insulin for Dynamic Control of Apoptosis in Serum-Free Culture of Chinese Hamster Ovary Cells (ANIMAL BIOTECHNOLOGY)
- Combined Addition of Glutathione and Iron Chelators for Decrease of Intracellular Level of Reactive Oxygen Species and Death of Chinese Hamster Ovary Cells
- Effect of Antioxidants on the Apoptosis of CHO Cells and Production of Tissue Plasminogen Activator in Suspension Culture
- In Vitro Proliferation of Primary Rat Hepatocytes Expressing Ureogenesis Activity by Coculture with STO Cells
- Effects of High Concentrations of Energy Sources and Metabolites on Suspension Culture of Chinese Hamster Ovary Cells Producing Tissue Plasminogen Activator
- Effect of Mixing Time on Taxoid Production Using Suspension Cultures of Taxus chinensis in a Centrifugal Impeller Bioreactor
- Effect of pore diameter of a porous membrane on progenitor cell content during membrane-separated coculture of hematopoietic cells and a stromal cell line
- Starch Particles Modified with Gelatin as Novel Small Carriers for Mammalian Cells
- Nitrogen depletion for intracellular triglyceride accumulation to enhance liquefaction yield of marine microalgal cells into a fuel oil
- Effect of Hydrostatic Pressure on Hybridoma Cell Metabolism
- Effect of Temperature on Cell Population Balance in Dexter's Culture of Murine Bone Marrow Hematopoietic Cells with Stromal Cells
- Selective Retension of Active Cells Employing Low Centrifugal Force at the Medium Change during Suspension Culture of Chinese Hamster Ovary Cells Producing tPA
- Acetone-Butanol Fermentation by Clostridium aurantibutyricum ATCC 17777 from a Model Medium for Palm Oil Mill Efflluent
- Membrane Localization of Transporter Associated with Antigen Processing (TAP)-Like (ABCB9) Visualized in Vivo with a Fluorescence Protein-Fusion Technique(Biochemistry/Molecular Biology)
- Effect of salt concentration on intracellular accumulation of lipids and triacylglyceride in marine microalgae Dunaliella cells.
- Effect of Concentrations of Murine Stromal and Hematopoietic Cells on the Progenitors Expansion in Their Three-Dimensional Coculture in Nonwoven Fabrics
- Rational Design for Stabilization and Optimum pH Shift of Serine Protease AprN
- Nucleotide Sequence and Possible Functional Mechanism of the Transcriptional Activator Gene (preL) for Neutral Protease from Lactobacillus sp.
- Nucleotide Sequence of the Neutral Protease Gene (nprL) from Lactobacillus sp. and Characterization of the Enzyme
- Purification and Characterization of a New Metal Protease Which Hydrolyzes the Cyclic Decapeptide, Gramicidin S
- Cloning and Nucleotide Sequence of the Regulatory Gene, degM, for Minor Serine Protease in Baciilus subtilis
- Role of the Pre-Pro-Region of Neutral Protease in Secretion in Bacillus subtilis
- Antibacterial Activity and Characteristics of Modified Ferrite Powder Coated with a Gemini Pyridinium Salt Molecule
- Correlation between the Bacterioclastic Action of a Bis-quaternary Ammonium Compound and Outer Membrane Proteins
- Construction of a Leftover Bath Water Model for Microbial Testing
- Characterization and Protein Analysis of Escherichia coli Resistant to a Quaternary Ammonium Compound
- Intermedilysin Is Essential for the Invasion of Hepatoma HepG2 Cells by Streptococcus intermedius
- Synthesis and Antimicrobial Characteristics of a Novel Biocide, 4, 4'-(1, 6-Dioxyhexamethylene)bis-(1-alkylpyridinium halide)
- Synthesis and Antimicrobial Characteristics of Novel Biocides, 1, 1'-(Decanedioyl)bis(4-methyl-4-alkylpiperazinium iodide)s with a Gemini Structure
- Synthesis and Antimicrobial Characteristics of Novel Antimicrobial Agent, 1,4-Dialky-1,4-dihydroxyethylpiperadinium Diiodides Immobilized on Inorganic Materials
- Antimicrobial Characteristics of Bis-quaternary Ammonium Compounds Possessing a p-Phenylene Group in Their Spacer Chains
- Kinetic Analysis of the Bactericidal Action of a Bis-quaternary Ammonium Compound against Escherichia coli
- Bactericidal Action of a Bis-puaternary Ammonium Compound against Vegetative Cells and Spores of Bacillus subtilis
- Characterization of Pseudomonas aeruginosa Resistant to a Quaternary Ammonium Compound
- Synthesis and Antimicrobial Characteristics of 4,4',4''-(1,2,3-Propylenetrithio)tris-(1-alkylpyridinium iodide)s
- Control of Legionella Species and Host Amoeba by Bis-quaternary Ammonium Compounds
- Bactericidal Action of 4,4'-(α, ω-Polymethylenedithio)bis-(1-alkylpyridinium iodide)s
- Synthesis and Antimicrobial Characteristics of 4,4'-(α, ω-Polymethylenedithio)bis(1-alkylpyridinium iodide)s
- Synthesis and Antibacterial Characteristics of N-Alkyl-4-Allylthiopyridinium Bromides
- Quantitative Structure-Activity Relationship of Antibacterial Dodecylpyridinium lodide Derivatives
- Bactericidal Action of N-Alkylcyanopyridinium Bromides against Escherichia coli K12 W3110
- Purification and Characterization of the Nitrilase from Alcaligenes faecalis ATCC 8750 Responsible for Enantioselective Hydrolysis of Mandelonitrile
- Microbial Contamination in Home Laundry Operations in Japan
- The Properties of Bacterial Adaptation to Mono- and Bis-Quaternary Ammonium Compounds
- Bacterioclastic Action of a Bis-Quaternary Ammonium Compound against Escherichia coli
- Bactericidal Action of a Bis-puaternary Ammonium Compound against Vegetative Cells and Spores of Bacillus subtilis