Bactericidal Action of a Bis-puaternary Ammonium Compound against Vegetative Cells and Spores of Bacillus subtilis

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概要

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• The bactericidal actions of a novel bis-QAC, 4, 4'-(1, 6-hexamethylenedithio) bis (1-octylpyridinium iodide) (4DTBP-6, 8) against the vegetative cells and the spores of<I> Bacillus subtilis</I> ATCC6633 were investigated. The bactericidal activity (log MBC<SUP>-1</SUP>) did not change in the temperature range of 10-50°C, the pH range of 5.0-9.0 and the contact time over 5min but remarkably depended on the initial cell concentration and the recovery medium. It was found that lecithin contributed to the recovery of the injured bacteria besides inactivating 4DTBP-6, 8 whereas Tween 80 had no effect. Furthermore, 4DTBP-6, 8 exhibited a strong inhibition against the germination and the outgrowth of spores. On the other hand, the kinetics analysis of the bactericidal action revealed that the initial death rate constant (<I>k</I>) for the vegetative cells was much higher than that for the spores. However, the initial death rate constant for the vegetative cells was dependent on both the temperature and pH while this was not the case for the spores. The activation energy (<I>E</I><SUB>a</SUB>) for the vegetative cells rapidly decreased with the increasing concentration of 4DTBP-6, 8, and the value of <I>E</I><SUB>a</SUB> (1.97kJ/mol) at the concentration of 9 μM was similar to that (2.10kJ/mol) for the spores at the concentration of 100 μM. The difference the concentration exponent (<I>n</I>) between the vegetative cells and the spores was observed, such as <I>n</I>≅1 for the vegetative cells and <I>n</I><0.2 for the spores at low temperatures. These results indicate that the spores are much more resistant to 4DTBP-6, 8 than the vegetative cells and suggest that the kinetics of the bactericidal action of 4DTBP-6, 8 against the vegetative cells and the spores are different.

• 日本防菌防黴学会の論文
• 2003-09-20

著者

• Takagi Mutsumi

  Pharmaceutical Research And Development Department Asahi Chemical Industry Company Ltd.:(present Add

• Maeda Takuya

  Department Of Biotechnology Faculty Of Eigineering Osaka University

• Maeda Takuya

  Department Of Biological Science And Technology Faculty Of Engineering The University Of Tokushima

• Takagi M

  Japan Advanced Inst. Sci. And Technol. Ishikawa Jpn

• NAGAMUNE Hideaki

  Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima

• Kourai H

  Department Of Biological Science And Technology Biosystems Engineering Institute Of Technology And S

• Kourai Hiroki

  Department Of Biological Science And Technology Faculty Of Engineering The University Of Tokushima

• Maeda T

  Laboratory Of Biochemistry And Molecular Biology Graduate School Of Pharmaceutical Sciences Osaka Un

• Nagamune Hideaki

  Department Of Biological Science And Technology Faculty Of Engineering The University Of Tokushima

• Zhang Daxin

  Department Of Biological Science And Technology Faculty Of Engineering The University Of Tokushima

• Maeda Takuya

  Department of Agricultural Chemistry, University of Osaka Prefecture

• Kourai Hiroki

  Department of Applied Chemistry, Technical College of Tokushima University

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