Small heat shock protein AgsA : An effective stabilizer of enzyme activities(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
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概要
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A small heat shock protein, AgsA, possesses chaperone activity that can reduce the amount of heat-aggregated protein in vivo, and suppress the aggregation of chemical- and heat-denatured proteins in vitro. Therefore, we examined the ability of AgsA to stabilize the activity of several enzymes by using this chaperone activity. We observed that AgsA can stabilize the enzymatic activities of Renilla (Renilla reniformis) luciferase, firefly (Photinus pyralis) luciferase, and β-galactosidase, and showed comparable or greater stabilization of these enzymes than bovine serum albumin (BSA), a well-known stabilizer of enzyme activities. In particular, AgsA revealed better stabilization of Renilla luciferase and β-galactosidase than BSA under disulfide bond-reducing conditions with dithiothreitol. In addition, AgsA also increased the enzymatic performance of β-galactosidase and various restriction enzymes to a comparable or greater extent than BSA. These data indicate that AgsA may be useful as a general stabilizer of enzyme activities.
- 公益社団法人日本生物工学会の論文
- 2013-01-00
著者
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Whiley Robert
Department Of Clinical And Diagnostic Oral Sciences Barts And The London Queen Mary's School Of
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Tabata Atsushi
Department Of Biological Science And Technology Faculty Of Engineering The University Of Tokushima
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Ishikawa Yoko
Department Of Chemistry University Of Tsukuba
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Nagamune Hideaki
Department Of Biological Science And Technology Faculty Of Engineering The University Of Tokushima
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Whiley Robert
Department of Clinical and Diagnostic Oral Sciences, Institute of Dentistry, Bart's and The London School of Medicine and Dentistry, Queen Mary, University of London
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Tomoyasu Toshifumi
Department of Biological Science and Technology, Institute of Technology and Science, The University of Tokushima Graduate School
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Ishikawa Yoko
Department of Biological Science and Technology, Institute of Technology and Science, The University of Tokushima Graduate School
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Tomoyasu Toshifumi
Department of Biological Science and Technology, Institute of Technology and Science, The University of Tokushima Graduate School:Department of Resource Circulation Engineering, Center for Frontier Research of Engineering, The University of Tokushima Grad
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Tabata Atsushi
Department of Biological Science and Technology, Institute of Technology and Science, The University of Tokushima Graduate School
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Nagamune Hideaki
Department of Biological Science and Technology, Institute of Technology and Science, The University of Tokushima Graduate School
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- Small heat shock protein AgsA : An effective stabilizer of enzyme activities(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Small heat shock protein AgsA : An effective stabilizer of enzyme activities