Crystallization and Preliminary X-Ray Diffraction Studies of Bacterial Alginate Lyase
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概要
- 論文の詳細を見る
Alginate lyase (EC 4.2.2.3) from a soil bacterium (Flavobacterium sp.), which was overexpressed in Bacuillus subtilis, has been crystallized by the vapor-diffusion method. Tetragonal crystals were obtained using ammonium sulfate as a precipitant. The crystal was resistant to X-ray radiation damage and diffracted to about 2.2A resolution. The diffraction pattern indicated that the crystal belongs to the orthorhomibic system, space group P2_12_12_1 with unit-cell dimensions of a=82.7,b=142.1 and c=80.9 Å. It is supposed that the asymmetric unit consists of two alginate lyase molecules.
- 社団法人日本生物工学会の論文
- 1994-06-25
著者
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TAKAGI Mutsumi
International Center for Biotechnology, Osaka University
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Inoue T
Tokyo Inst. Technol. Yokohama Jpn
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Kimura A
Kyoto Univ. Kyoto
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IMANAKA Tadayuki
Department of Synthetic and Biological Chemistry, Graduate School of Engineering, Kyoto University
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TAKAGI MASAHIRO
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Kimura A
Hokkaido Univ. Sapporo Jpn
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Takagi M
Asahi Chemical Industry Co. Ltd. Shizuoka Jpn
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Maeda Takuya
Department Of Biotechnology Faculty Of Eigineering Osaka University
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Imanaka Tadayuki
Department Of Applied Biotechnology Faculty Of Engineering Osaka University
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HISANO TOMOHIRO
Department of Biotechnology, Kyoto Institute, Gunze Co. Ltd.,
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MIKAMI BUNZO
Departments of New Food Design
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INOSE TOMOKO
Applied Microbiology
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SAKAGUCHI KENJI
Research Institute for Food Science, Kyoto University
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KIMURA AKIRA
Applied Microbiology
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MURATA KOUSAKU
Applied Microbiology
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Yamashita Tetsuo
Food & Beverage Division Otsuka Chemical Co.
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Yamashita Tesuo
The Third Department Of Internal Medicine Hamamatsu University School Of Medicine
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Kimura A
Research Institute For Food Science Kyoto University
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Takagi M
Japan Advanced Inst. Sci. And Technol. Ishikawa Jpn
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Yamashita T
Hiroshima Univ. Hiroshima Jpn
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Sakaguchi Kenji
Research Institute For Food Science Kyoto University
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松下 一信
山口大 農
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Takagi Masahiro
Department Of Biotechnology Faculty Of Engineering Osaka University
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