Effect of Temperature on Cell Population Balance in Dexter's Culture of Murine Bone Marrow Hematopoietic Cells with Stromal Cells
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概要
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The effect of temperature in the range from 25 to 37℃ on the population balance of stromal and multiplelineage hematopoietic cells from murine bone marrow at various stages of differentiation in Dexter's culture was investigated. The length of time required for stromal cells to reach confluence after inoculation of both cell types harvested from murine bone marrow was shorter at higher temperatures. On the other hand, the hematopoietic cell concentration initially decreased more rapidly at higher temperatures until the confluence of stromal cells, which should then support the proliferation of hematopoietic cells. The concentration of hematopoietic cells began to increase after 2 weeks incubation at 33 and 37℃ and after 4 weeks at 29℃. However, the growth of hematopoietic cells was not stable at 37℃, and neither stromal nor hematopoietic cells showed significant growth at 25℃. The specific growth rate of hematopoietic cells at 29℃ after 4 weeks was comparable or higher than that at 33℃, while the final concentration of hematopoietic cells was maximal at 33℃. Using a cultivation method in which hematopoietic cells were recharged onto a confluent layer of stromal cells prepared at 33℃, the maintenance and growth of hematopoietic cells were better at 29℃ than at higher temperatures. The content of progenitor cells among the hematopoietic cells increased prior to the increase in thehematopoietic cell concentration, and the progenitor cell was greater content at the lower temperature. These results suggest that cultivation at 29℃ might be superior to 33℃ for the maintenance and growth of hematopoietic cells with a prepared confluent layer of stromal cells.
- 社団法人日本生物工学会の論文
- 1999-08-25
著者
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TAKAGI Mutsumi
International Center for Biotechnology, Osaka University
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YOSHIDA Toshiomi
International Center for Biotechnology, Osaka University
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Yoshida Toshiomi
International Center For Biotechnology Osaka University
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Takagi Mutsumi
Internatioal Center For Biotechnology Osaka University
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KUBOMURA DAIKI
International Center for Biotechnology, Osaka University
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Kubomura Daiki
International Center For Biotechnology Osaka University
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Yoshida Toshiomi
Internatioal Center For Biotechnology Osaka University
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