Characterization of Multi-Cellulases of Sporotrichum cellulophilum by Using Monoclonal Antibodies
スポンサーリンク
概要
- 論文の詳細を見る
Cellulases of Sporotrichum cellulophilum, CII1,CII2,CII3,CII5,CIII1,CIII2,and CIV1,were purified to homogeneity by column chromatographies and preparative electrophoresis. The specific activity of CII3 was the highest (100 units/mg), while the activities of CII5,CIII1,and CII2 were low (12-17 units/mg). The molecular weight of CII2 was 19,000,while those of the others ranged from 46,000-63,000. All the cellulases were glycoproteins except for CII2. Three monoclonal antibodies (Mab CII11,Mab CII12,and MabCII13) were prepared against CII1,and Mab CIV11 against CIV1. From the immunological reactivities, it was found that CII1,CIII2,and CIV1 had a common antigenic site recognized by Mab CII11,Mab CII12,and Mab CII13,while CIII2 and CVI1 had a different antigenic site that was recognized by Mab CIV11. MabCII11,MabCII12,MabCII13,and MabCIV11 did not react with CII2,CII3,CII5,and CIII1. The classification of these cellulases was discussed.
- 公益社団法人日本生物工学会の論文
- 1991-03-25
著者
-
Yoshida Toshiomi
International Center For Biotechnology Osaka University
-
KINOSHITA SHINICHI
International Center of Cooperative Research in Biotechnology, Faculty of Engineering, Osaka Univers
-
Okuno K
Suntory Inst. Medicinal Res. And Dev. Gunma Jpn
-
OKUNO KAZUAKI
International Center of Cooperative Research in Biotechnology, Faculty of Engineering, Osaka Univers
-
SAWAMURA KEISUKE
International Center of Cooperative Research in Biotechnology, Faculty of Engineering, Osaka Univers
-
Yoshida Toshiomi
International Center Of Cooperative Research In Biotechnology Faculty Of Engineering Osaka Universit
-
Sawamura Keisuke
International Center Of Cooperative Research In Biotechnology Faculty Of Engineering Osaka Universit
-
Kinoshita Shinichi
International Center Of Cooperative Research In Biotechnology Faculty Of Engineering Osaka Universit
-
Kinoshita Shinichi
International Center Of Cooperative Research In Biotechnology Faculty Of Engineering Osaka Universit
-
Yoshida Toshiomi
Internatioal Center For Biotechnology Osaka University
関連論文
- Effect of heparin addition on expansion of cord blood hematopoietic progenitor cells in three-dimensional coculture with stromal cells in nonwoven fabrics
- Analysis of Hematopoietic Microenvironment Containing Spatial Development of Stromal Cells in Nonwoven Fabrics (ANIMAL BIOTECHNOLOGY)
- Construction of Xylose-Assimilating Saccharomyces cerevisiae
- Transduction of Static Pressure Signal to Expression of Human Granurocytes Macrophage Colony Stimulating Factor Mrna in Chinese Hamster Ovary Cells (ANIMAL BIOTECHNOLOGY)
- Effect of Static Pressure on Human Granulocyte-Macrophage Stimulating Factor (hGM-CSF) Production by Chinese Hamster Ovary Cells
- Effects of Shifts Up and Down in Osmotic Pressure on Production of Tissue Plasminogen Activator by Chinese Hamster Ovary Cells in Suspension
- Effect of chondroitin sulfate and hyaluronic acid on gene expression in a three-dimensional culture of chondrocytes
- Effect of Poly DL-Lactic-Co-Glycolic Acid Mesh on a Three-Dimensional Culture of Chondrocytes(CELL AND TISSUE ENGINEERING)
- Hydrodynamically stable adhesion of endothelial cells onto a polypropylene hollow fiber membrane by modification with adhesive protein
- Enhanced adhesion of endothelial cells onto a polypropylene hellow-fiber membrane by plasma discharge treatment and high inoculum cell density
- Characterization of Recombinant Human Man_9-Mannosidase Expressed in Escherichia coli
- A Mass Spectrometry Membrane Probe and Practical Problems Associated with Its Application in Fermentation Processes
- On-Line Monitoring and Control of Aceotone-Butanol Fermentation by Membrane-Sensor Mass Spectrometry
- Application of the Fuzzy Theory to Simulation of Batch Fermentation
- 413 Simulation of Fermentation Process by Using Fuzzy Set Theory
- Metabolic Flux Change in Hybridoma Cells under High Osmotic Pressure
- Global and Local Neural Network Models in Biotechnology : Application to Different Cultivation Processes
- 519 Control of Acetic Acid Accumulation during Cultivation of Recombinant E__-. coli AT2471.
- Characteristic of the Oligosaccharide Structure of Jack Bean α-Mannosidase with Some Lectins
- Production of Monascus Pigments by a Solid-Liquid State Culture Method
- Physiologically Motivated Strategies for Control of the Fed-Batch Cultivation of Recombinant Escherichia coli for Phenylalanine Production
- Effect of Sugar Residues in a Glycolipid Coated onto a Dish on Ammonia Consumption and Gluconeogenesis Activity of Primary Rat Hepatocytes
- Synthesis and Evaluation of Novel Peptide-Lipid
- Effect of galactose residue in glycolipid coated onto a dish on ammonia consumption activity of primary rat hepatocytes
- Expanded Bed Adsorption for Purification of Alcohol Dehydrogenase Using a Dye-Iminodiacetic Acid Matrix (BIOCHEMICAL ENGINEERING)
- Multivalent Binding Interaction of Alcohol Dehydrogenase on Dye-Metal Affinity Matrix(BIOCHEMICAL ENGINEERING)
- Development of New Dye-Metal Agarose-Coated Alumina Matrix and Elution Strategy for Purification of Alcohol Dehydrogenase
- 1116 Dye-metal affinity chromatography in mixed mode for purification of alcohol dehydrogenase
- 336 Development of polyethyleneimine-coated alumina for dye-affinity chromatography
- Continuous Ethanol Production by a Concentrated Culture of Flocculating Yeast
- Control of Water Content in a Solid-State Culture of Aspergillus oryzae
- An Ingenious Device for Better Prediction of Bio-Processes Using a Multiple Regression Model : A case Study on Glucose Isomerase Production
- An Expert Approach for Control of Fermentation Processes as Variable Structure Plants
- Repeated Addition of Insulin for Dynamic Control of Apoptosis in Serum-Free Culture of Chinese Hamster Ovary Cells (ANIMAL BIOTECHNOLOGY)
- Combined Addition of Glutathione and Iron Chelators for Decrease of Intracellular Level of Reactive Oxygen Species and Death of Chinese Hamster Ovary Cells
- Effect of Antioxidants on the Apoptosis of CHO Cells and Production of Tissue Plasminogen Activator in Suspension Culture
- Isolation of an Alginate-Degrading Organism and Purification of Its Alginate Lyase
- In Vitro Proliferation of Primary Rat Hepatocytes Expressing Ureogenesis Activity by Coculture with STO Cells
- Glucose Feeding Strategy Accounting for the Decreasing Oxidative Capacity of Recombinant Escherichia coli in Fed-Batch Cultivation for Phenylalanine Production
- Dewatering of Extracellular Polymer and Activated Sludges by Thermochemical Treatment
- Properties of Extracellular Polymer Having an Effect on Expression of Activated Sludge
- Dewatering Characteristics of Activated Sludges and Effect of Extracellular Polymer
- Computer-Aided On-Line Monitoring of Physiological Variables in Suspended Cell Cultures of Perilla frutescens in a Bioreactor
- Effects of High Concentrations of Energy Sources and Metabolites on Suspension Culture of Chinese Hamster Ovary Cells Producing Tissue Plasminogen Activator
- Effect of Mixing Time on Taxoid Production Using Suspension Cultures of Taxus chinensis in a Centrifugal Impeller Bioreactor
- Effect of pore diameter of a porous membrane on progenitor cell content during membrane-separated coculture of hematopoietic cells and a stromal cell line
- Starch Particles Modified with Gelatin as Novel Small Carriers for Mammalian Cells
- Nitrogen depletion for intracellular triglyceride accumulation to enhance liquefaction yield of marine microalgal cells into a fuel oil
- Effect of Hydrostatic Pressure on Hybridoma Cell Metabolism
- Effect of Temperature on Cell Population Balance in Dexter's Culture of Murine Bone Marrow Hematopoietic Cells with Stromal Cells
- Selective Retension of Active Cells Employing Low Centrifugal Force at the Medium Change during Suspension Culture of Chinese Hamster Ovary Cells Producing tPA
- Acetone-Butanol Fermentation by Clostridium aurantibutyricum ATCC 17777 from a Model Medium for Palm Oil Mill Efflluent
- Knowledge-Based Design and Operation of Bioprocess Systems
- Effect of salt concentration on intracellular accumulation of lipids and triacylglyceride in marine microalgae Dunaliella cells.
- Effect of Concentrations of Murine Stromal and Hematopoietic Cells on the Progenitors Expansion in Their Three-Dimensional Coculture in Nonwoven Fabrics
- HPLC Monitoring System for Lactic Acid Fermentation
- 323 Purification of Cellulases from Sporotrichum cellulophilum and Existence of Multienzyme Components
- Xanthan Gun Production in a Fermentor with Twin Impellers
- Enhanced Glutamic Acid Production of Brevibacterium sp. with Temperature Shift-Up Cultivation(BREWING AND FOOD TECHNOLOGY)
- Mechanistic Analysis of Xanthan Gum Production in a Stirred Tank
- Effect of Glucose Concentration on Xanthan Gum Production by Xanthomonas campestris
- Effects of Temperature on Cell Growth and Anthocyanin Production in Suspension Cultures of Perilla frutescens
- Characterization of Multi-Cellulases of Sporotrichum cellulophilum by Using Monoclonal Antibodies
- Enzymatic Hydrolysis of Sweet Potato for Energy-Saving Production of Ethanol
- Lactic Acid Production by a Filter-Bed-Type Reactor
- Adaptive Control Strategies for the Mashing Process in Sake Brewing
- Circulation time distribution and volume of mixing regions in highly viscous xanthan gum solution in a stirred vessel.
- Effect of galactose residue in glycolipid coated onto a dish on ammonia consumption activity of primary rat hepatocytes
- Effects of agitation by flat-bladed turbine impeller on microbial production of xanthan gum.