Repeated Addition of Insulin for Dynamic Control of Apoptosis in Serum-Free Culture of Chinese Hamster Ovary Cells (ANIMAL BIOTECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
Repeated addition of insulin, together with an initial addition of the reduced form of glutathione (GSH) and a chelator, aurintricarboxylic acid (ATA), was proposed as a strategy for the dynamic control of apoptosis in serum-free culture of Chinese hamster ovary (CHO) cells. Insulin suppressed cell death in serum-free culture by means of lowering intracellular reactive oxygen species levels via an increase in the specific glucose consumption rate. This effect of insulin addition was found not only at 0 h but also at 48h during serum-free cultivation. In consideration of the reduction of insulin by GSH, insulin was again added at 24h following the initial addition during the serum-free cultivation. The repeated addition of insulin decreased cell death and increased the production of tissue plasminogen activator compared with the single addition at 0h.
- 社団法人日本生物工学会の論文
- 2003-07-25
著者
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TAKAGI Mutsumi
International Center for Biotechnology, Osaka University
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YOSHIDA Toshiomi
International Center for Biotechnology, Osaka University
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Yoshida Toshiomi
大阪大学生物工学国際交流センター
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Yoshida Toshiomi
International Center Of Cooperative Research In Biotechnology Faculty Of Engineering Osaka Universit
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Yoshida T
International Center For Biotechnology Osaka University
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Yoshida Toshiomi
International Center For Biotechnology Osaka University
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吉田 豊和
岐阜大・工・生命工
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Takagi Mutsumi
International Center For Biotechnology Osaka University:(present Address)division Of Biotechnology A
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Takagi M
International Center For Biotechnology Osaka University
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Takagi M
Japan Advanced Inst. Of Sci. And Technol. Ishikawa Jpn
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Takagi M
Osaka Univ. Osaka Jpn
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Yoshida T
Kinki Univ. Nara Jpn
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Yoshida Toyokazu
Dept. Food Nutri. Fac. Agr. Kinki Univ.
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Takagi Mutsumi
Internatioal Center For Biotechnology Osaka University
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Takagi Mutsumi
School Of Materials Science Japan Advanced Institute Of Science And Technology
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Yun Zhanyou
International Center For Biotechnology Osaka University
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Yoshida T
Department Of Biomolecular Science Gifu University
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Yoshida Toshiomi
Internatioal Center For Biotechnology Osaka University
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吉田 豊和
岐阜大 工
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