Production of Bacterial Alginate-Specific Lyase by Recombinant Bacillus subtilis
スポンサーリンク
概要
- 論文の詳細を見る
Conditions for the production of bacterial alginate-specific lyase (A1-III) by Bacillus subtilis transformed with an inducible secretion vector pISA412 harboring the A1-III gene from a bacterium (Flavobacterium sp.) of strain A1 were studied. Galactose at around 3% was found as the most efficient carbon source in the pH region between 7.0〜8.5. A higher amount of potassium phosphate was required to repress degradation of A1-III produced in medium. Under the most preferable culture conditions determined, production of the lyase reached approximately 0.3 mg/ml. The properties of A1-III purified from the medium were comparable with those of A1-III present in strain A1 in terms of molecular size, substrate specificity and in N-terminal amino acid sequence.
- 社団法人日本生物工学会の論文
- 1994-07-25
著者
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TAKAGI Mutsumi
International Center for Biotechnology, Osaka University
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MURATA KOUSAKU
Research Institute for Food Science, Kyoto University
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KIMURA AKIRA
Research Institute for Food Science, Kyoto University
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Kimura A
Kyoto Univ. Kyoto
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Kimura Akira
Research Institute For Food Science Kyoto University
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IMANAKA Tadayuki
Department of Synthetic and Biological Chemistry, Graduate School of Engineering, Kyoto University
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TAKAGI MASAHIRO
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Kimura A
Hokkaido Univ. Sapporo Jpn
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Takagi M
Asahi Chemical Industry Co. Ltd. Shizuoka Jpn
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Maeda Takuya
Department Of Biotechnology Faculty Of Eigineering Osaka University
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Imanaka Tadayuki
Department Of Applied Biotechnology Faculty Of Engineering Osaka University
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HISANO TOMOHIRO
Department of Biotechnology, Kyoto Institute, Gunze Co. Ltd.,
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NISHIMURA MINORU
Department of Biotechnology, Kyoto Institute, Gunze Co. Ltd.,
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YAMASHITA TETSUO
Division of Food & Beverage Research, Otsuka Chemical Co. Ltd.,
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Yamashita Tetsuo
Food & Beverage Division Otsuka Chemical Co.
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Yamashita Tesuo
The Third Department Of Internal Medicine Hamamatsu University School Of Medicine
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Murata Kousaku
Research Institute For Food Science Kyoto Univeristy
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Kimura A
Research Institute For Food Science Kyoto University
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Takagi M
Japan Advanced Inst. Sci. And Technol. Ishikawa Jpn
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Nishimura Minoru
Department Of Biotechnology Kyoto Institute Gunze Co. Ltd.
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Yamashita T
Hiroshima Univ. Hiroshima Jpn
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Sakaguchi Kenji
Research Institute For Food Science Kyoto University
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松下 一信
山口大 農
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Takagi Masahiro
Department Of Biotechnology Faculty Of Engineering Osaka University
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Kimura Akira
Research Center for Solar Energy Chemistry, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan
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